STUDIES OF ONCOGENE EXPRESSION DURING CELLULAR GROWTH AND DIFFERENTIATION

细胞生长和分化过程中癌基因表达的研究

• 批准号: 4692398
• 负责人: J FALCO
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4692398
• 关键词: cell differentiation; developmental genetics; embryo /fetus cell /tissue; epidermal growth factor; gene expression; growth media; mammalian embryology; oncogenes; placenta; skin; transforming virus

Proto-oncogene expression was studied in the BALB-MK epithelial cell line following mitogenic stimulation with epidermal growth factor (EGF). The following results were obtained: C-fos expression: C-fos is undetectable in total cellular RNA of quiescent cells prior to addition of EGF. After addition of EGF, c-fos expression rises sharply to a sharp peak, one hour post-stimulation. It then drops off rapidly to low but detectable levels within the next few hours. C-myc expression: These cells express constitutively high levels of c-myc when quiescent. When EGF is added, c-myc expression rises four- to eight-fold to a plateau beginning at two hours and ending at eight hours post-stimulation. It then steadily declines to steady-state levels higher than prior to addition of EGF. Effects of TPA: The tumor promoter TPA was found to mimic the mitogenic effects of EGF and, like EGF, to stimulate a sharp one-hour peak of c-fos when given to BALB-MK cells in the absence of EGF. Ca++ and c-fos expression: Ca++, which is a signal for terminal differentiation in BALB-MK cells, was found to cause a decline in c-fos expression.

原癌基因在BALB-MK上皮细胞系中的表达 在用表皮生长因子(EGF)刺激有丝分裂后。这个 取得了以下成果： C-fos表达：静止期细胞总RNA中未检测到c-fos 加入EGF前的细胞。加入EGF后c-fos的表达 在刺激后一小时，急剧上升到一个急剧的峰值。然后它就会下降 在接下来的几个小时内迅速降至低但可检测到的水平。 C-myc表达：这些细胞结构性高表达c-myc 在静止时。当加入EGF时，c-myc的表达增加了4- 从两小时开始到八小时结束的八倍到平台期 刺激后。然后，它稳步下降到更高的稳态水平 比加入EGF前更高。 TPA的作用：肿瘤促进剂TPA被发现模拟有丝分裂原 EGF的作用和EGF一样，刺激c-fos的一个小时高峰 在没有EGF的情况下给予BALB-MK细胞。 Ca++和c-fos表达：Ca++，是终端的信号 在BALB-MK细胞中的分化可导致c-fos的下降 表情。