SERUM-FREE CULTURE OF MOUSE KERATINOCYTES

小鼠角质细胞的无血清培养

• 批准号: 3963485
• 负责人: J FALCO
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3963485
• 关键词: cell bank /registry; cell differentiation; developmental genetics; embryo /fetus cell /tissue; epidermal growth factor; gene expression; growth media; insulin; keratinocyte; mammalian embryology; molecular biology; oncogenes; oncogenic virus; placenta; skin; tissue /cell culture; transforming virus

A serum-free defined media system was developed for the BALB/MK mouse kertinocyte cell line. It was found that this cell line required only two growth factors, insulin and epidermal growth factor (EGF), for growth rates which approached those achievable in serum-containing media supplemented with EGF. This defined media system was then used to determine the precise growth factor requirements of BALB/MK cells transformed with a variety of oncoviruses. It was found that nearly all of the viral transformants were able to grow in the absence of EGF. However, different oncoviruses varied in how efficiently they abrogated EGF dependence. H-ras-, K-ras-, fgr- and abl-transformed cells became entirely EGF-independent. Fms-, mos-, and erbB-transformed cells grew in the absence of EGF, but grew better when EGF was present. Cells transformed with fgr and mos were also able to grow in the absence of EGF. This defined media system has also been adapted as a method of stringently selecting for cells which escape from growth factor requirements following transformation.

建立了BALB/MK小鼠无血清成分确定培养基体系 角质形成细胞系 发现该细胞系仅需要两个 生长因子，胰岛素和表皮生长因子（EGF），用于生长速率 这接近那些在含有血清的培养基中可实现的， EGF 然后使用该确定培养基系统来确定 用各种不同的细胞因子转化的BALB/MK细胞的因子需求 肿瘤病毒 发现几乎所有的病毒转化体都是 可以在没有EGF的情况下生长。 然而，不同的肿瘤病毒 如何有效地消除对EGF的依赖 H-ras-、K-ras-、fgr-和 abl-transformed细胞成为完全EGF非依赖性。 Fms-、mos-和 erbB转化的细胞在没有EGF的情况下生长，但当EGF 在场 用fgr和mos转化的细胞也能在 没有EGF。 这种定义媒体系统也被改编为一种严格的方法， 选择逃避生长因子需求的细胞， 转型