ENHANCEMENT OF IMMUNOGENICITY OF CAPSULAR POLYSACCHARIDES PATHOGENIC BACTERIA

荚膜多糖病原菌免疫原性的增强

• 批准号: 4693830
• 负责人: R SCHNEERSON
• 金额: --
• 依托单位: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4693830
• 关键词: Haemophilus influenzae; bacterial antigens; bacterial polysaccharides; bactericidal immunity; cell capsule; gel electrophoresis; immunization; immunological substance; microorganism immunology; pilus

Adherence to mucous membranes is an important initial event in bacterial colonization and may be related to their pathogenicity. Pili, shown to be the adherence mechanism in several bacterial species, have been recently demonstrated in H. influenzae. Studies were initiated to isolate and purify hib pili, to evaluate their role in pathogenesis and immunity. Pili expression of several H. influenzae type b strains from disease isolates; CSF and epiglottitis and from carriers, was enriched utilizing their adherence properties to human RBC, as verified by EM. Two of these Hib strains were grown on solid media homoginized and pili isolated by several cycles of isoelectric precipitation followed by ammonium sulfate precipitation. Final purification utilized preparative gel electrophoresis. Rabbit antisera were prepared with these pili preparations.

粘附于粘膜是细菌感染的重要起始事件。 可能与其致病性有关。 皮利，被证明是 最近，几种细菌粘附机制 在H.流感。 研究开始分离和 纯化hib皮利，探讨其在致病和免疫中的作用。 皮利 几种H.来自疾病分离株的流感B型菌株; CSF和会厌炎以及携带者，利用其 粘附特性，如通过EM验证的。 将这些Hib菌株中的两种在均质化的固体培养基上生长，并将皮利 通过几个循环的等电沉淀分离， 硫酸铵沉淀。 最终纯化采用制备型 凝胶电泳 用这些皮利制备兔抗血清 准备工作