ENHANCEMENT OF IMMUNOGENICITY OF CAPSULAR POLYSACCHARIDES PATHOGENIC BACTERIA

黏附于粘膜是细菌的一个重要的初始事件 定殖性，可能与它们的致病性有关。菌毛，显示为 几种细菌的黏附机制是最近研究的 在流感嗜血杆菌中得到证实。已经开始了分离和分离 纯化Hib菌毛，评价其在发病机制和免疫中的作用。毛发 病原菌中几株b型流感嗜血杆菌的表达 脑脊液和会厌炎以及从携带者那里获得的 与人红细胞的粘附性，经EM验证。 其中两株Hib菌株在均质和菌毛固体培养基上生长。 通过几个循环的等电沉淀分离出来，然后 硫酸铵沉淀法。利用制备剂进行最终提纯 凝胶电泳法。用这些菌毛制备兔抗血清 准备工作。

Adherence to mucous membranes is an important initial event in bacterial colonization and may be related to their pathogenicity. Pili, shown to be the adherence mechanism in several bacterial species, have been recently demonstrated in H. influenzae. Studies were initiated to isolate and purify hib pili, to evaluate their role in pathogenesis and immunity. Pili expression of several H. influenzae type b strains from disease isolates; CSF and epiglottitis and from carriers, was enriched utilizing their adherence properties to human RBC, as verified by EM. Two of these Hib strains were grown on solid media homoginized and pili isolated by several cycles of isoelectric precipitation followed by ammonium sulfate precipitation. Final purification utilized preparative gel electrophoresis. Rabbit antisera were prepared with these pili preparations.