AMMONIAGENESIS IN CULTURED RENAL (LLC-PK1) EPITHELIA

培养的肾 (LLC-PK1) 上皮细胞中的氨生成

• 批准号: 4694529
• 负责人: G GSTRAUNTHALER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4694529
• 关键词: acidosis; ammonia; cell bank /registry; epithelium; gluconeogenesis; glutamine; growth media; kidney metabolism; renal tubule acidosis

Ammoniagenesis was studied in confluent cultures of the LLC-PK1 renal epithelial cell line. It was found that ammonia production was strictly dependent on the extracellular glutamine concentration. LLC-PK1 cells respond to metabolic acidosis. LLC-PK1 cells exhibited an increase in ammonia production and glutamine consumption with decreasing pH of the culture medium. In order to study the relationship between ammoniagenesis and gluconeogenesis attempts have been undertaken to isolate LLC-PK1 cells capable of gluconeogenesis.

在LLC-PK 1肾细胞的汇合培养物中研究了氨生成 上皮细胞系 结果发现，氨的生产是严格 依赖于细胞外谷氨酰胺浓度。 llc-pk 1细胞 对代谢性酸中毒有反应。 LLC-PK 1细胞表现出增加， 氨产量和谷氨酰胺消耗， 培养液 为了研究产氨作用与生物量的关系， 已经尝试分离LLC-PK 1细胞， 能够再生的