BRAIN SEROTONIN AND CARDIOVASCULAR CONTROL

大脑血清素和心血管控制

• 批准号: 4694641
• 负责人: R MCCARTY
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4694641
• 关键词: baroreceptors; blood pressure; brain; brain mapping; cardiovascular system; experimental brain lesion; heart rate; microinjections; neuropharmacology; neurotoxins; neurotransmitter metabolism; nitroferricyanide; phenylephrine; prosencephalon; serotonin; spinal cord mapping; spinal cord surgery

There is compelling evidence that central serotonin neurons are involved in regulation of the cardiovascular system. However, the brain circuitry which underlies this influence is poorly understood. The major focus of this project is to investigate the effects of discrete neurotoxic of brain serotonin pathways on baroreceptor reflexes in conscious rats. The selective serotonin neurotoxin, 5,7-dihydroxytryptamine (5,7-DHT), will be injected into: (a) NTS, (b) medial forebrain bundle, (c) spinal cord or (d) lateral ventricles. Vehicle-injected controls will be included for each group. Within two weeks following microinjections of 5,7-DHT or vehicle, rats will be prepared with chronic arterial and venous catheters to permit direct recordings of blood pressure and heart rate and administration of drugs into conscious, unrestrained rats. Baroreceptor function will be assessed by dose-response curves for sodium nitroprus-side (0.5-20 Mug/kg) in each rat. Our findings to date indicate that baroreceptor function is largely spared following localized or global brain serotonin lesions.

有令人信服的证据表明，中枢5-羟色胺神经元参与 调节心血管系统。 然而，大脑回路 这种影响的基础是什么，人们知之甚少。 的主要重点 本项目旨在研究离散性脑神经毒性物质 5-羟色胺通路对清醒大鼠压力感受器反射的影响 的 选择性5-羟色胺神经毒素，5，7-二羟色胺（5，7-DHT），将被 注射到：（a）NTS，（B）内侧前脑束，（c）脊髓或 (d)侧脑室 将纳入溶剂注射对照品， 每组 在微量注射5，7-DHT或 溶剂，将用慢性动脉和静脉导管制备大鼠 允许直接记录血压和心率， 给清醒的、不受约束的大鼠施用药物。 压力感受 将通过硝普钠侧的剂量反应曲线评估功能 （0.5-20 μ g/kg）。 我们迄今为止的发现表明， 压力感受器功能在局部或整体脑 血清素损伤