AUTORADIOGRAPHIC ANALYSIS OF SOMATOSTATIN BINDING SITES IN BRAIN

脑中生长抑素结合位点的放射自显影分析

• 批准号: 4694642
• 负责人: R MCCARTY
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4694642
• 关键词: autoradiography; brain mapping; brain metabolism; brain septal area; chemical binding; densitometry; medial olfactory area; neuropharmacology; online computer; radiotracer; somatostatin

The biologically active peptide, somatostatin, has been localized in several hypothalamic and extrahypothalamic brain regions where it may function as a classical neurotransmitter or as a modulator of neural activity. We have studied somatostatin binding sites in rat brain by incubation of tissue sections with 125-I-tyr-l-somatostatin, Ultrofilm autoradiography, computerized microdensitometry and comparison with 125-I standards. Highest concentrations of somatostatin binding sites (fmol/mg protein) were found in the claustrum, central nucleus of the amygdala, deep layers of cerebral cortex, lateral olfactory nuclei, hippocampus, medial and lateral septal nuclei, and the medial habenula. Regulation of somatostatin binding sites may be studied as one approach to examining the involvement of brain somatostatin pathways in various pysiological and behavioral states.

具有生物活性的肽，生长抑素，已经定位于 几个下丘脑和下丘脑外脑区， 作为经典的神经递质或作为神经递质的调节剂， 活动 我们研究了大鼠脑内生长抑素的结合位点， 用125-I-tyr-l-生长抑素孵育组织切片，Ultrofilm 放射自显影、计算机化显微密度测定及与125-I的比较 标准 生长抑素结合位点的最高浓度（fmol/mg 在屏状核、杏仁核中央核、杏仁核深部核、杏仁核中央 大脑皮层、外侧嗅核、海马、内侧 外侧隔核和内侧缰核。 调控 生长抑素结合位点可以作为一种方法来研究， 脑生长抑素通路参与各种生理和 行为状态