AUTORADIOGRAPHIC ANALYSIS OF SOMATOSTATIN BINDING SITES IN BRAIN

生物活性多肽生长抑素已经定位于 下丘脑和下丘脑外的几个脑区 起经典神经递质或神经调节剂的作用 活动。我们研究了大鼠脑内生长抑素的结合部位。 ~(125)I-酪氨酸-L-生长抑素超薄膜孵育组织切片 放射自显影、计算机显微密度测定及其与125-I的比较 标准。生长抑素结合位点的最高浓度(fmoL/mg 蛋白质)位于屏状核、杏仁中央核、深部 大脑皮层、嗅外核层、海马层、内侧层 外侧隔核和内侧缰核。规管 生长抑素结合部位可以作为一种检查 脑生长抑素通路参与多种生理和病理过程 行为状态。

The biologically active peptide, somatostatin, has been localized in several hypothalamic and extrahypothalamic brain regions where it may function as a classical neurotransmitter or as a modulator of neural activity. We have studied somatostatin binding sites in rat brain by incubation of tissue sections with 125-I-tyr-l-somatostatin, Ultrofilm autoradiography, computerized microdensitometry and comparison with 125-I standards. Highest concentrations of somatostatin binding sites (fmol/mg protein) were found in the claustrum, central nucleus of the amygdala, deep layers of cerebral cortex, lateral olfactory nuclei, hippocampus, medial and lateral septal nuclei, and the medial habenula. Regulation of somatostatin binding sites may be studied as one approach to examining the involvement of brain somatostatin pathways in various pysiological and behavioral states.