EXCITATORY AMINOACID RECEPTOR FUNCTION IN CEREBELLAR GRANULE CELLS

小脑颗粒细胞的兴奋性氨基酸受体功能

• 批准号: 4696428
• 负责人: F NICOLETTI
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4696428
• 关键词: aminophosphonate; aspartate; brain cell; brain metabolism; cyclic GMP; fatty acids; glutamates; human subject; inositol; ionic strengths; kainate; lipid metabolism; microglia; phosphatidylinositols; phospholipids; tissue /cell culture

We have used primary cultures of cerebellar granule cells to study the intracellular molecular processes elicited by the interaction of excitatory amino acids with specific membrane receptors. We have shown that at least two different dicarboxylic amino acids receptors are coupled with inositol phospholipid metabolism in primary cultures of cerebellar granule cells; one is sensitive to the stimulatory action of glutamate and aspartate and is preferentially antagonized by 2-amino-5-phosphonovalerate (APV); the other is activated by kainic acid and preferentially antagonized by cis, 2,3-piperidindicarboxylic acid (PDA). The activation of these sites by glutamate, asfertate and kainic acid also results in an enhanced formation of cGMP. The stimulatory action of glutamate and aspartate on cGMP is markedly influenced by the ionic composition of the incubation buffer and is potentiated by depolarizing stimuli. The action of kainic acid is independent of such parameters.

我们用原代培养的小脑颗粒细胞来研究 细胞内的分子过程引起的相互作用的兴奋性 氨基酸与特定的膜受体。 我们已经证明，至少 两种不同的二羧酸氨基酸受体与肌醇偶联 原代培养小脑颗粒细胞的磷脂代谢; 一个对谷氨酸和天冬氨酸的刺激作用敏感， 优先被2-氨基-5-膦酰基戊酸（APV）拮抗; 另一种被红藻氨酸激活并优先被顺式拮抗， 2，3-哌啶二甲酸（PDA）。 这些网站的激活， 谷氨酸盐、阿司弗酸盐和红藻氨酸也导致增强的形成 cGMP。 谷氨酸和天冬氨酸对cGMP的刺激作用是 显著受孵育缓冲液的离子组成影响， 是通过去极化刺激增强的 红藻氨酸的作用是 与这些参数无关。