BIOLOGICAL MODIFICATION OF HUMAN GLIOMA CELLS IN VITRO

糖胺多聚糖(GAG)是多阴离子化合物，通常结合在一起。 共价连接到蛋白质核心。它们是细胞的重要组成部分。 并与细胞与细胞之间的相互作用有关。人脑胶质瘤细胞株的研究 组织培养产生的透明质酸(HA)比 正常的神经胶质细胞，它们正在将其在介质中脱落。还有，一定的 胶质瘤细胞系正在形成对透明质酸酶敏感的“保护膜” 这削弱了它们诱导同种异体CTL反应的能力。我们正在使用 糖皮质激素(地塞米松)或二甲基亚砜(DMSO)抑制 体外培养脑胶质瘤细胞合成透明质酸和其他GAG 目的使他们更容易受到免疫反应和/或 化疗。由于地塞米松可以用于人类，因此 改良术在治疗神经胶质瘤患者中可能是有用的。

Glycosaminoglycans (GAG) are polyanionic compounds, usually bound covalently to a protein core. They are a prominent component of the cell surface and are implicated in cell-cell interaction. Human glioma cells in tissue culture produce a much greater amount of hyaluronic acid (HA) than normal glial cells and they are shedding it in the media. Also, certain glioma cell lines are forming a hyaluronidase-sensitive "protective' coat that impairs their ability to elicit allogeneic CTL response. We are using glucocorticoids (Dexamethasone) or dimethylsulfoxide (DMSO) to inhibit the biosynthesis of HA and other GAG in cultured glioma cells with the objective to make them more susceptible to immune response and/or chemotherapy. Since Dexamethasone can be administered to humans, such a modification may be useful in the management of patients with gliomas.