本研究目的是应用现代影像学手段在分子水平观察C6胶质瘤细胞发生，发育，繁殖，代谢机制和癌细胞的传播方式。方法是拟以双编码慢病毒载体转染技术，即以编码有增强型的绿色荧光蛋白（EGFP）报告基因和磁共振成像（MRI）的报告基因【携带铁蛋白重链（H-ferritin）】双启动子慢病毒为载体转染C6大鼠胶质瘤细胞为基础，制备特定的C6大鼠胶质瘤细胞株（原癌基因c-myc被标记表达）和相应的动物模型，采用免疫荧光光学成像和MRI技术，对EGFP的荧光强度和myc-hFTH磁共振驰豫效率的进行测定和比较，结合神经病理组织染色和HE染色，定性和定量地观察和分析比较胶质瘤细胞株c-myc基因体内外表达的特征，特别是胶质瘤细胞巢和新生瘤血管生物学分布的分子影像学特征。期望在进一步提高认识胶质瘤发病机制的同时，为实现胶质瘤的早期诊断和鉴别诊断以及评价基因治疗疗效，寻求一类新型的内源性MR生物"对比剂"。

The aim of this study was to apply modern imaging method to observe the C6 glioma cells occurs, develop, breeding, metabolic mechanism and the spread way of cancer cells in the molecular level. Based on bimodal lentiviral vector transfection technology, we try to develop a bimodal lentiviral vector to monitor deep tissue events using MRI to detect the C6 rats glioma cell myc-tagged human ferritin heavy chain (myc-hFTH) expression and fluorescence imaging to detect enhanced green fluorescent protein (EGFP) expression in the specific C6 rats glioma cell lines (having myc-hFTH) and the corresponding animal models. The both imaging parameters, i.e. the fluorescence intensity of the EGFP for immunofluorescence optical imaging, and the relaxation intensity (T2*, R2* )of the myc-hFTH for MRI, will be compared and analysis. The both imaging characteristics will be matched with the data from the neural histopathological staining and HE dyed slices. Through the qualitative and quantitative analysis and comparison of the results delivered from the C6 rats glioma cell lines with myc-hFTH gene and EGFP expressions in vivo and in vitro, especially the features of the glioma cell nests and the distribution of the new tumor vascular, it is hopeful to further understanding mechanism of gliomas and realizing the development of glioma, being able to have the early diagnosis and differential diagnosis and evaluating gene therapy. It is expected to seek a new type of endogenous MR biological "contrast agents" as well.