DIFFERENTIATION AND REGULATION OF GENE EXPRESSION IN GLIAL CELLS

星形胶质细胞应该是一个撇号，分化和功能是 在蛋白质和基因水平上进行研究。 为了识别星形胶质细胞特异性 标记物、质膜、核和核基质蛋白， 通过二维电泳解析，并与 神经元、胶质瘤C6细胞和肾成纤维细胞。 两丰膜 蛋白质被鉴定，纯化至均一，并将被 以抗体为特征的。 来描述大脑的进化 在胶质瘤中，我们检测了胶质细胞酸性蛋白（GFAP）的表达， 一种星形胶质细胞标记物，在正常和转化的人类星形胶质细胞中。 在这些 细胞GFAP似乎受到翻译后修饰的调节， 其组装似乎受GTP控制。 胶质纤维酸性蛋白在恶性胶质瘤和 胎儿星形胶质细胞的电荷相似，而在良性胶质瘤细胞中， 是带负电的 脑谷氨酰胺合成酶是氮代谢的关键酶， 定位于星形胶质细胞，在发育和神经系统中受到调节。 五 cDNA克隆在小鼠脑文库中被鉴定，该文库与一个 克隆仓鼠谷氨酰胺合成酶基因。 我们将用这些克隆人 研究谷氨酰胺合成酶基因的转录调控。

Astrocytes should be an apostrophe differentiation and function are investigated at protein and gene levels. To identify astrocyte-specific markers, plasma membrane, nuclear, and nuclear matrix proteins were resolved by two-dimensional electrophoresis and compared to those in neurons, glioma C6 cells, and kidney fibroblasts. Two abundant membrane proteins were identified, purified to homogeneity, and will be characterized by means of antibodies. To characterize evolution of brain gliomas, we examined expression of glial fibrillary acidic protein (GFAP), an astrocyte marker, in normal and transformed human astrocytes. In these cells GFAP appeared to be regulated by post-translational modification and its assembly seemed to be controlled by GTP. GFAP in malignant gliomas and fetal astrocytes were similarly charged, while in benign glioma cells it was more electronegative. Brain glutamine synthetase, a key enzyme in nitrogen metabolism and localized in astrocytes, is regulated developmentally and hormonally. Five cDNA clones were identified in a mouse brain library that hybridized with a cloned hamster glutamine synthetase gene. We will use these clones to study the transcriptional regulation of the glutamine synthetase gene.