Cryo-Electron Microscopy Research Infrastructure

冷冻电子显微镜研究基础设施

• 批准号: MC_PC_17137
• 负责人: Yvonne Jones
• 金额: $ 462.66万
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Intramural
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MC_PC_17137
• 关键词: Cryo; Electron; Microscopy; Research; Infrastructure

The Oxford Centre for Particle Imaging at the Wellcome Trust Centre for Human Genetics is pivotal for pan-departmental access and training in biological electron cryo microscopy (cryoEM) and electron cryo tomography (cryoET) for the 300+ structural biology research community in Oxford. State-of-the-art cryoEM and cryoET can image the molecular mechanisms by which human cells, disease pathogens and vaccines work. Such studies can pinpoint how differences in the genetic makeup of individuals contribute to a wide range of medical conditions through effects at the cellular and molecular level. Likewise, the design of vaccines to combat emergent viruses can be guided by mapping the surface details of the virus that are best recognised by our immune system. This grant application is to upgrade instrumentation for state-of-the-art cryoEM and cryoET in OPIC. OPIC isunique in Europe in having the capability, when necessary, to run cryoEM and cryoET data collection in containment. OPIC, through its association with the UK national facility, eBIC (electron Bio-Imaging Centre) at Diamond Light Source, provides access for such experiments to the broader national and international user community. On-going analyses on viruses and vaccines important to human health worldwide will benefit from the requested equipment.

在惠康信托中心的粒子成像牛津中心人类遗传学是至关重要的泛部门的访问和培训生物电子冷冻显微镜（cryoEM）和电子冷冻断层扫描（cryoET）为300+结构生物学研究社区在牛津。最先进的cryoEM和cryoET可以对人类细胞、疾病病原体和疫苗的分子机制进行成像。这些研究可以查明个体基因组成的差异如何通过细胞和分子水平的影响导致广泛的医疗状况。同样，对抗新出现病毒的疫苗设计可以通过绘制我们免疫系统最能识别的病毒表面细节来指导。这项拨款申请是为了升级OPIC中最先进的cryoEM和cryoET仪器。OPIC在欧洲是独一无二的，在必要时，有能力在安全壳内运行cryoEM和cryoET数据收集。OPIC通过其与联合王国国家设施-钻石光源电子生物成像中心-的联系，向更广泛的国家和国际用户社区提供进行此类实验的机会。正在进行的对全世界人类健康至关重要的病毒和疫苗分析将受益于所要求的设备。

项目成果

Structures and therapeutic potential of anti-RBD human monoclonal antibodies against SARS-CoV-2.

• DOI: 10.7150/thno.65563
• 发表时间: 2022
• 期刊: Theranostics
• 影响因子: 12.4
• 作者: Huang KA;Zhou D;Tan TK;Chen C;Duyvesteyn HME;Zhao Y;Ginn HM;Qin L;Rijal P;Schimanski L;Donat R;Harding A;Gilbert-Jaramillo J;James W;Tree JA;Buttigieg K;Carroll M;Charlton S;Lien CE;Lin MY;Chen CP;Cheng SH;Chen X;Lin TY;Fry EE;Ren J;Ma C;Townsend AR;Stuart DI
• 通讯作者: Stuart DI

Phospho-regulation, nucleotide binding and ion access control in potassium-chloride cotransporters.

• DOI: 10.15252/embj.2020107294
• 发表时间: 2021-07-15
• 期刊: The EMBO journal
• 作者: Chi G;Ebenhoch R;Man H;Tang H;Tremblay LE;Reggiano G;Qiu X;Bohstedt T;Liko I;Almeida FG;Garneau AP;Wang D;McKinley G;Moreau CP;Bountra KD;Abrusci P;Mukhopadhyay SMM;Fernandez-Cid A;Slimani S;Lavoie JL;Burgess-Brown NA;Tehan B;DiMaio F;Jazayeri A;Isenring P;Robinson CV;Dürr KL
• 通讯作者: Dürr KL

A conserved glutathione binding site in poliovirus is a target for antivirals and vaccine stabilisation.

• DOI: 10.1038/s42003-022-04252-5
• 发表时间: 2022-11-25
• 期刊: Communications biology
• 影响因子: 5.9

Structure, mechanism, and inhibition of Hedgehog acyltransferase.

• DOI: 10.1016/j.molcel.2021.11.018
• 发表时间: 2021-12-16
• 期刊: Molecular cell
• 影响因子: 16
• 作者: Coupland CE;Andrei SA;Ansell TB;Carrique L;Kumar P;Sefer L;Schwab RA;Byrne EFX;Pardon E;Steyaert J;Magee AI;Lanyon-Hogg T;Sansom MSP;Tate EW;Siebold C
• 通讯作者: Siebold C

The antigenic anatomy of SARS-CoV-2 receptor binding domain.

• DOI: 10.1016/j.cell.2021.02.032
• 发表时间: 2021-04-15
• 期刊: Cell
• 影响因子: 64.5
• 作者: Dejnirattisai W;Zhou D;Ginn HM;Duyvesteyn HME;Supasa P;Case JB;Zhao Y;Walter TS;Mentzer AJ;Liu C;Wang B;Paesen GC;Slon-Campos J;López-Camacho C;Kafai NM;Bailey AL;Chen RE;Ying B;Thompson C;Bolton J;Fyfe A;Gupta S;Tan TK;Gilbert-Jaramillo J;James W;Knight M;Carroll MW;Skelly D;Dold C;Peng Y;Levin R;Dong T;Pollard AJ;Knight JC;Klenerman P;Temperton N;Hall DR;Williams MA;Paterson NG;Bertram FKR;Siebert CA;Clare DK;Howe A;Radecke J;Song Y;Townsend AR;Huang KA;Fry EE;Mongkolsapaya J;Diamond MS;Ren J;Stuart DI;Screaton GR
• 通讯作者: Screaton GR