MECHANISMS OF CELLULAR IMMUNE RESPONSES

我们的研究继续强调T细胞黏附和 迁移，特别是当它们与 血管内皮细胞和相关的组织迁移。我们的研究已经 揭示了一种有说服力的、强大的、快速的细胞F- 肌动蛋白：解聚到远低于先前观察到的水平 相反，机械刺激导致肌动蛋白在 不到1秒。我们假设机械感应是必不可少的。 对细胞骨架重排、运动和细胞的调节 黏附，包括白细胞与内皮细胞的结合。我们有 系统地研究了趋化因子产生的调控 内皮细胞，并发现规则符合 用于从底层传输信息的放大系统 组织传给了白细胞。我们已经确定了成纤维细胞的特征 网状(FRC)系统，我们将其解释为专门的系统 用于将信息传递给淋巴结中的高内皮细胞。 我们发现了系统内的可溶性调解人和丰富的证据 细胞外基质决定因素的显示最有可能 促进淋巴结内淋巴细胞的移动。我们终于有了 研究了内皮细胞和T细胞上的原糖链。的 在这些研究中，我们特别注意到，尽管软骨素 硫酸盐在T细胞上的表达水平较低，而硫酸软骨素 对T淋巴细胞侵入胶原胶很重要。

Our studies continue to emphasize studies of T cell adhesion and migration, particularly as they relate to interactions with endothelium and related migration into tissue. Our studies have revealed a persuasive, powerful and rapid regulator of cellular F- actin:to depolymerize to levels far below those previously observed and conversely mechanical stimulation causes actin repolymerization in less than 1 second. We hypothesize that mechanosensation is essential to the regulation of cytoskeletal rearrangement, locomotion and cell adhesion, including leukocyte binding to endothelium. We have systematically studied the regulation of chemokine production by endothelial cells and find the rules fit the properties of an amplification system for conveying information from the underlying tissue to passing leukocytes. We have characterized the fibroblastic reticular (FRC) system, which we interpret to be a specialized system for conveying information to the high endothelial cells in lymph node. We find evidence both for soluble mediators within the system and rich display of extracellular matrix determinants which most likely facilitate lymphocyte movement within lymph node. Finally we have investigated protoglycan both on endothelial cells and on T cells. Of particular note in those studies, we find that although chondriotin sulfate is expressed at low level on T cells, that chondroitin sulfate is important for the invasion of T lymphocytes into collagen gels.