EFFECTS OF LIPID PEROXIDATION PRODUCT 4 HYDROXY 2 NONENAL ON CULTURED CELLS

脂质过氧化产物4羟基2壬烯醛对培养细胞的影响

• 批准号: 5203483
• 负责人: K FANG
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5203483
• 关键词: adduct; aging; aldehydes; carbonyl group; human tissue; lipid peroxides; model; tissue /cell culture; vascular endothelium

Free radicals and reactive oxygen species readily interact with polyunsaturated fatty acids in cell membranes resulting in the formation of cytotoxic aldehydes. These aldehydes are considerably more stable than free radicals and thus are likely to diffuse into the cellular medium. Among these aldehydes, 4-hydroxy-2-nonenal (HNE) is one of the major products of membrane peroxidation and shows many biological effects. Lipid peroxidation increases during aging and in many diseases, including atherosclerosis. Modification of proteins and other biomolecules by lipid peroxidation products is therefore believed to have a significant role in senescence and in some diseases. The mechanisms by which HNE acts are not, however, well understood. Human Aortic Endothelial Cell (HAEC) was chosen as a model for studying the effects of HNE on human endothelium. Incubation of HAEC with HNE resulted in an increase in the level of protein carbonyl groups. The formation of HNE-protein adduct in the cell upon the treatment of HNE is presently under investigation. The results of this and related experiments may provide better understanding on the role of lipid peroxidation in disease- and age-related accumulation of damaged protein and impairment of cellular function.

自由基和活性氧容易与 细胞膜中的多不饱和脂肪酸， 有细胞毒性的醛。 这些醛类比 因此很可能扩散到细胞内 介质 在这些醛中，4-羟基-2-壬烯醛（HNE）是其中一种 膜过氧化的主要产物，并显示出许多生物 方面的影响. 脂质过氧化作用在衰老过程中增加， 疾病，包括动脉粥样硬化。蛋白质的修饰和其他 因此，认为脂质过氧化产物引起的生物分子 在衰老和某些疾病中起着重要作用。 的 然而，HNE作用的机制还没有很好地理解。 以人主动脉内皮细胞（HAEC）为模型， HNE对人内皮细胞的影响。 HAEC与HNE的孵育 导致蛋白质羰基水平的增加。 的 HNE处理后细胞中HNE-蛋白质加合物的形成 目前正在接受调查 这一结果和相关的 实验可能会提供更好的了解脂质的作用， 与疾病和年龄相关的受损蛋白质积累中的过氧化作用 和细胞功能受损。