EFFECTS OF LIPID PEROXIDATION PRODUCT 4 HYDROXY 2 NONENAL ON CULTURED CELLS

自由基和活性氧物种很容易与 细胞膜中多不饱和脂肪酸的形成 细胞毒性醛。这些醛要稳定得多。 因此很可能会扩散到细胞内 5~6成熟。在这些醛中，4-羟基-2-壬烯醛(HNE)是一种 膜过氧化的主要产物，并显示出许多生物 效果。脂质过氧化在衰老过程中增加，在许多 疾病，包括动脉粥样硬化。蛋白质和其他物质的修饰 因此，由脂质过氧化产物产生的生物分子被认为 在衰老和某些疾病中起着重要作用。这个 然而，HNE的作用机制还没有被很好地理解。 以人主动脉内皮细胞(HAEC)为研究对象 HNE对人血管内皮细胞的影响HAEC与HNE的孵育 导致蛋白质的羰基水平增加。这个 HNE处理后细胞内HNE蛋白加合物的形成 目前正在接受调查。这一结果和相关的 实验可能会对脂质的作用有更好的理解 疾病和年龄相关的受损蛋白质积累中的过氧化作用 以及细胞功能受损。

Free radicals and reactive oxygen species readily interact with polyunsaturated fatty acids in cell membranes resulting in the formation of cytotoxic aldehydes. These aldehydes are considerably more stable than free radicals and thus are likely to diffuse into the cellular medium. Among these aldehydes, 4-hydroxy-2-nonenal (HNE) is one of the major products of membrane peroxidation and shows many biological effects. Lipid peroxidation increases during aging and in many diseases, including atherosclerosis. Modification of proteins and other biomolecules by lipid peroxidation products is therefore believed to have a significant role in senescence and in some diseases. The mechanisms by which HNE acts are not, however, well understood. Human Aortic Endothelial Cell (HAEC) was chosen as a model for studying the effects of HNE on human endothelium. Incubation of HAEC with HNE resulted in an increase in the level of protein carbonyl groups. The formation of HNE-protein adduct in the cell upon the treatment of HNE is presently under investigation. The results of this and related experiments may provide better understanding on the role of lipid peroxidation in disease- and age-related accumulation of damaged protein and impairment of cellular function.