CORE--PEPTIDE SYNTHESIS AND PROTEIN SEQUENCING AND ANALYSIS

肽合成和蛋白质和分析核心将提供服务 并在肽合成领域为RCOB研究人员提供建议， 蛋白质测序和己糖胺和氨基酸分析。 蛋白 在Porton Model PI 2090 E肽上进行微测序 用于鉴定PTH-氨基的使用在线HPLC的微测序系统 acids. 每个循环释放的残留物的鉴别由以下方法辅助： 叠加或减去连续PTH-氨基酸的计算机软件 分居 肽合成在Rainin Model P53上使用 FMOC固相自动化肽化学。 肽从 树脂载体和由反相HPLC判断的纯度 分居 己糖胺和氨基酸分析使用沃茨 PicoTag系统。 项目3将主要使用核心 第七，偶尔也会有第六个项目。 核心设备，波顿 PI 2090 E型肽微测序系统，Rainin P53型肽 合成仪和Perkin-Elmer（系列4）HPLC最近已获得 其他资金来源。

The Peptide Synthesis and Protein and Analysis Core will provide service and advice to RCOB investigators in the area of peptide synthesis, protein sequencing and hexosamine and amino acid analysis. Protein Microsequencing is performed on a Porton Model PI 2090E peptide microsequencing system using on-line HPLC for identification of PTH-amino acids. Identification of the residue released at each cycle is aided by computer software which overlays or subtracts successive PTH-amino acid separations. Peptide Synthesis is performed on a Rainin Model P53 using FMOC solid phase automated peptide chemistry. Peptide are cleaved from the resin support and the purity judged from a reverse-phase HPLC separation. Hexosamine and Amino Acid Analysis is done using the Waters PicoTag system. The Core will be used to a major extent by Projects 3 and 7 and occasionally by Project 6. Equipment for the core, Porton Model PI 2090E peptide microsequencing system, Rainin Model P53 peptide synthesizer and Perkin-Elmer (Series 4) HPLC have recently been acquired from other funding sources.