CORE--PEPTIDE SYNTHESIS AND PROTEIN SEQUENCING AND ANALYSIS

多肽合成和蛋白质分析核心将提供服务 以及对RCOB研究人员在多肽合成领域的建议， 蛋白质测序和氨基己糖和氨基酸分析。蛋白 对Porton Model PI 2090E多肽进行微测序 在线高效液相色谱微测序系统鉴定PTH-氨基 酸。在每个循环中释放的残留物的识别由 覆盖或减去连续的PTH-氨基酸的计算机软件 分居。在Rainin模型P53上进行多肽合成 Fmoc固相自动多肽化学。多肽是从 从反相高效液相色谱判断树脂载体和纯度 分离。己糖胺和氨基酸分析是用水进行的 PicoTag系统。核心将在很大程度上被项目3使用 和7，偶尔通过项目6。核心设备，波顿 PI2090E型多肽微测序系统、Rainin型P53多肽 Synsizer和Perkin-Elmer(Series 4)高效液相色谱仪最近已被收购 从其他资金来源。

The Peptide Synthesis and Protein and Analysis Core will provide service and advice to RCOB investigators in the area of peptide synthesis, protein sequencing and hexosamine and amino acid analysis. Protein Microsequencing is performed on a Porton Model PI 2090E peptide microsequencing system using on-line HPLC for identification of PTH-amino acids. Identification of the residue released at each cycle is aided by computer software which overlays or subtracts successive PTH-amino acid separations. Peptide Synthesis is performed on a Rainin Model P53 using FMOC solid phase automated peptide chemistry. Peptide are cleaved from the resin support and the purity judged from a reverse-phase HPLC separation. Hexosamine and Amino Acid Analysis is done using the Waters PicoTag system. The Core will be used to a major extent by Projects 3 and 7 and occasionally by Project 6. Equipment for the core, Porton Model PI 2090E peptide microsequencing system, Rainin Model P53 peptide synthesizer and Perkin-Elmer (Series 4) HPLC have recently been acquired from other funding sources.