MOLECULAR PHYLOGENY OF INDO-PACIFIC CORAL REEF FISHES

这项拟议的工作的目的是辨别出 珊瑚礁鱼类中最似是而非的一科--豆瓣鱼 (Pomacentridae)，通过继续扩展生化遗传数据集 已在关岛大学(UOG)海军陆战队组装 实验室。具体地说，预计拟议的研究 将导致积累足够的电泳谱数据 有必要对现有的形态基础进行评估 印度-西太平洋的种、亚属和属的分类 豆腐鱼。拟议工作的一个辅助目标是确保 UOG海洋实验室继续支持新兴的 生物化学和分子遗传学领域：实现这一目标可以 只会增强我们提供研究生和本科生的能力 为有意从事职业生涯的学生提供分子生物学培训 生命科学和卫生专业。据预计， 拟议的研究计划将大幅扩大数据库，该数据库 由我们先前对豆娘鱼属的电泳图调查得出 Pomacentus、Stestes和金翅目。来自以下地点的电泳数据 这三个属的其他代表将被添加到 数据库，并在构建进化树时使用 基于频率或简约的分析。从基因上推断 然后将系统发育与现有的形态进行比较 这些分类群的系统模式。拟议研究的意义 如果看到成果，它将：1)验证 将分子生物学技术引入到珊瑚礁鱼类研究领域，即 至关重要，因为珊瑚礁群落正在变得危险 受人类活动影响的警戒率；2)提供了许多 学生在分子生物学方面所需的培训机会 技巧。

The aim of the proposed work is to discern the molecular systematics of one of the most specious families of coral reef fishes, the damselfishes (Pomacentridae), by continued expansion of a biochemical genetic data set that has been assembled at the University of Guam (UOG) Marine Laboratory. Specifically, it is anticipated that the proposed research will result in the accumulation of sufficient electrophoretic data necessary for the evaluation of the existing morphologically based classification of species, subgenera, and genera of Indo-Western Pacific damselfishes. An ancillary aim of the proposed work is to ensure that the UOG Marine Laboratory continues to support research in the burgeoning fields of biochemical and molecular genetics: achievement of this aim can only result in our enhanced ability to offer graduate and undergraduate training in molecular biology to students intending to pursue careers in the life sciences and health professions. It is anticipated that the proposed plan of research will expand substantially the data base which resulted from our prior electrophoretic surveys of the damselfish genera Pomacentrus, Stegastes, and Chrysiptera. Electrophoretic data from additional representatives of these three genera will be added to the data base and used in the construction of evolutionary trees by either frequency- or parsimony- based analyses. Genetically inferred phylogenies will then be compared with the existing morphologic systematic scheme for these taxa. The significance of the proposed study is that, if seen to fruition, it would: 1) validate the application of molecular biological techniques to an area of reef fish research that is crucial, given that coral reef communities are becoming imperiled at an alarming rate by the effects of human activities and; 2) provide much needed opportunities for student training in molecular biological techniques.