Developing high throughput single cell proteomics

揭示组织或细胞群体中蛋白质表达的可变性对于理解细胞如何对其环境做出反应至关重要。例如，这可以提供细胞对感染或化疗反应的详细信息。到目前为止，单细胞蛋白分析已经触手可及，但其应用仍然繁琐且通量低。本提案旨在开发和部署一种简单而可靠的方法来分析单细胞中的蛋白质含量。

Unraveling the variability in the protein expression in tissues or cell populations is critical to understand how cells respond to their environment. For example, this can provide details about the cellular response to an infection or to a chemoterapic. As of today, single cell protein analyses has become at reach, but its application is still cumbersome and its throughput is low. This proposal aims to develop and deploy a simple and robust method to analyse the protein content in single cells.