Establishment of the Cambridge Single Cell Analysis Clinical Core Facility [SCACCF]

建立剑桥单细胞分析临床核心设施 [SCACCF]

• 批准号: MR/M008975/1
• 负责人: Patrick Maxwell
• 金额: $ 443.13万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FM008975%2F1
• 关键词: Establishment; Cambridge; Single; Cell; Analysis

Recent technology developments have revolutionised our ability to characterize, quantify and isolate single cells. Examination of diseases at single cell resolution, both at diagnosis and after treatment, will transform the practice of molecular medicine by improving the quality of patient diagnosis, refining treatment options, monitoring of response to treatment, and detecting the emergence of resistance to treatment. Cambridge scientists have been at the forefront of basic research in single cell expression profiling and the analysis of circulating tumour DNA, as well as setting up local Biotech companies that develop novel single cell technologies. Here we propose to use MRC Infrastructure funding to create a new shared core facility for single cell analysis (the Cambridge Single Cell Analysis Clinical Core Facility [SCACCF]), which will serve all major molecular medicine programmes in Cambridge: cancer, neurosciences, immunity and inflammation, infectious diseases, stem cell and regenerative medicine, metabolic medicine and experimental therapeutics. SCACCF will be well placed to act as a hub for the UK and will work closely with our outstanding strategic partners in the Cambridge area (Babraham Institute, Wellcome Trust Sanger Institute, the European Bioinformatics Institute, the MRC Laboratory for Molecular Biology, major pharma & biotech), to bring their different capabilities to bear on clinical research challenges.

最近的技术发展彻底改变了我们表征、量化和分离单细胞的能力。在诊断时和治疗后以单细胞分辨率检查疾病，将通过提高患者诊断质量、完善治疗方案、监测治疗反应以及检测治疗耐药的出现来改变分子医学的实践。剑桥大学的科学家一直处于单细胞表达谱和循环肿瘤 DNA 分析基础研究的前沿，并建立了开发新型单细胞技术的本地生物技术公司。在这里，我们建议利用 MRC 基础设施资金创建一个新的单细胞分析共享核心设施（剑桥单细胞分析临床核心设施 [SCACCF]），该设施将为剑桥的所有主要分子医学项目提供服务：癌症、神经科学、免疫和炎症、传染病、干细胞和再生医学、代谢医学和实验疗法。 SCACCF 将充分发挥作为英国中心的作用，并将与我们在剑桥地区的杰出战略合作伙伴（Babraham 研究所、Wellcome Trust Sanger 研究所、欧洲生物信息学研究所、MRC 分子生物学实验室、主要制药和生物技术）密切合作，利用其不同的能力应对临床研究挑战。

项目成果

Neurogenin3 phosphorylation controls reprogramming efficiency of pancreatic ductal organoids into endocrine cells.

• DOI: 10.1038/s41598-018-33838-5
• 发表时间: 2018-10-18
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Azzarelli R;Rulands S;Nestorowa S;Davies J;Campinoti S;Gillotin S;Bonfanti P;Göttgens B;Huch M;Simons B;Philpott A
• 通讯作者: Philpott A

Pooled CRISPR-activation screening coupled with single-cell RNA-seq in mouse embryonic stem cells.

• DOI: 10.1016/j.xpro.2021.100426
• 发表时间: 2021-06-18
• 期刊: STAR protocols
• 作者: Alda-Catalinas C;Eckersley-Maslin MA;Reik W
• 通讯作者: Reik W

A nested cohort study of 6,248 early breast cancer patients treated in neoadjuvant and adjuvant chemotherapy trials investigating the prognostic value of chemotherapy-related toxicities.

• DOI: 10.1186/s12916-015-0547-5
• 发表时间: 2015-12-29
• 期刊: BMC medicine
• 影响因子: 9.3
• 作者: Abraham JE;Hiller L;Dorling L;Vallier AL;Dunn J;Bowden S;Ingle S;Jones L;Hardy R;Twelves C;Poole CJ;Pharoah PD;Caldas C;Earl HM
• 通讯作者: Earl HM

Lymphocyte density determined by computational pathology validated as a predictor of response to neoadjuvant chemotherapy in breast cancer: secondary analysis of the ARTemis trial.

• DOI: 10.1093/annonc/mdx266
• 发表时间: 2017-08-01
• 期刊: Annals of oncology : official journal of the European Society for Medical Oncology
• 作者: Ali HR;Dariush A;Thomas J;Provenzano E;Dunn J;Hiller L;Vallier AL;Abraham J;Piper T;Bartlett JMS;Cameron DA;Hayward L;Brenton JD;Pharoah PDP;Irwin MJ;Walton NA;Earl HM;Caldas C
• 通讯作者: Caldas C

Computational pathology of pre-treatment biopsies identifies lymphocyte density as a predictor of response to neoadjuvant chemotherapy in breast cancer.

• DOI: 10.1186/s13058-016-0682-8
• 发表时间: 2016-02-16
• 期刊: Breast cancer research : BCR
• 作者: Ali HR;Dariush A;Provenzano E;Bardwell H;Abraham JE;Iddawela M;Vallier AL;Hiller L;Dunn JA;Bowden SJ;Hickish T;McAdam K;Houston S;Irwin MJ;Pharoah PD;Brenton JD;Walton NA;Earl HM;Caldas C
• 通讯作者: Caldas C