PARALLEL TIME OF FLIGHT MASS SPECTROMETER

在药物发现应用中正在产生大量的化合物文库，这给分析科学家带来了越来越大的负担，他们需要在合成确认和更有前景的化合物的重点研究中快速表征这些样品。加剧了这一产能挑战的是，在聚合物珠上进行多步合成有机反应生成的化合物的纯度往往会受到影响。在没有层析分离的情况下，这些杂质可能会导致流动注射质谱仪(MS)中的假阳性和假阴性，这取决于分析物的物理化学性质、竞争中间体和树脂贡献的杂质。因此，负有MS职责的分析小组必须在(A)较快但信息量较少的流动注射方法和(B)较可靠但较慢的LC-MS方法之间进行选择。提出了一种显著提高MS样品吞吐量的方法，同时解决了只有LC-MS才能提供的高化学选择性的需求。利用专门的API飞行时间质谱仪的特性，可以同时对多个时变液体流进行质量分析，而不需要借助流动切换或任何其他时间切片方案。这种新的方法将允许多个LC系统耦合到单个API-TOF-MS，同时保持研究级别的优秀系数。拟议的商业应用：该设备将允许大量此类样品通过LC-MS进行表征，而无需部署额外的MS分析器。这种仪器将立即在那些高通量的筛选应用中找到用武之地，这些应用目前被分析仪器的连续样品引入所阻碍。与其说是一种研究工具，不如说是一种自动化工具，这种仪器非常适合于质量控制和制造操作中的自动化、高速、LC-MS。

Large compound libraries are being generated in pharmaceutical discovery applications, placing a growing burden on the analytical scientist to rapidly characterize these samples both at the gross scale for synthesis confirmation and in focused studies for more promising compounds. Exacerbating this throughput challenge, the purity of compounds generated fran multi-step synthetic organic reactions on polymeric beads is often compromised. In the absence of chromatographic separation, these impurities may lead to both false positives and false negatives in flow injection mass spectrometry (MS), depending upon the physio-chemical properties of the analyte, competing inter-mediate products and resin contributed impurities. Analytical groups with Ms responsibilities must therefore choose between (a) faster but less informative flow injection methods and (b) more reliable, but slower LC-MS methods. A method is proposed to deliver significantly increased Ms sample throughput, while at the same time addressing the need for high chemical selectivity which only LC-MS can deliver. Exploiting the characteristics of a specialized API time-of-flight mass spectrometer, multiple time-varying liquid streams will be simultaneously mass analyzed without resorting to flow switching or any other time-slice scheme. This new approach will allow multiple LC systems to be coupled to a single API-TOF-MS while maintaining research grade figures of merit. PROPOSED COMMERCIAL APPLICATION: This apparatus will allow significantly greater numbers of these samples to be characterized via LC-MS, without deploying additional MS analyzers. Such an instrument would find immediate utility in those high throughput screening applications which are presently bottle-necked by serial sample introduction to analytical instruments. Less a research tool than an automation tool, such an instruments would be ideally suited for automated, high speed, LC-MS in quality control and manufacturing operations.