ELECTROSPRAY ION TRAP TIME OF FLIGHT MASS SPECTROMETER

电喷雾离子阱飞行时间质谱仪

• 批准号: 2519761
• 负责人: JAMES G BOYLE
• 金额: $ 22.4万
• 依托单位: ANALYTICA OF BRANFORD, INC.
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2519761
• 关键词: biomedical equipment development; capillary electrophoresis; computer program /software; electrospray ionization mass spectrometry; gas chromatography; liquid chromatography; radiation detector

The concept of combining several independent mass analyzer instruments into,a single hybrid instrument has been exploited for many years and over many instrument combinations. Ion trap-time-of-flight (IT-TOF) is one of the most recent and potentially useful methods, combining very high sensitivity and fast scanning speed to serve as a generic detector for either high speed chromatography systems (gas, liquid) or electrophoresis systems (capillary). IT-TOF-MS is particularly attractive as a mass analysis method for atmospheric pressure ionization methods, since the ion trap device "buffers" the interaction between the API source and the TOF-MS device, both preserving sensitivity and enhancing mass spectral figures of merit. In the Phase I portion of this research, IT-TOF was demonstrated to have many physical and several analytical properties which distinguish it as a mass analysis tool. In addition to possessing very high sensitivity (by virtue of its ability to "store" ions in free space using radiofrequency waves), the device also retains much of its character as a TOF instrument, allowing detection over an unlimited mass range and acquisition of full mass spectra in quasi real-time. The Phase II portion of this research will focus on the refinement of the technique as an analytical method, concentrating on systematic performance enhancements. A prototype instrument will be built for the demonstration of applications requiring very high sensitivity as well as very high scanning speed in conjunction with either liquid chromatography or capillary electrophoresis. PROPOSED COMMERCIAL APPLICATION: The subject apparatus will be a "second generation" time-of-flight MS interface for high speed separations systems using Electrospray and other atmospheric pressure ionization technologies. It will combine ultra-high sensitivity for sample limited applications, as well as high resolution and high mass accuracy for demanding biological analyses.

将多个独立的质量分析仪器组合在一起的概念 一个单一的混合仪器已经开发了很多年， 多种乐器组合。离子阱飞行时间（IT-TOF） 最新的和潜在的有用的方法之一，结合非常 灵敏度高、扫描速度快，可用作通用检测器 用于高速色谱系统（气体、液体）或 电泳系统（毛细管）。IT-TOF-MS特别有吸引力 作为用于大气压电离方法的质量分析方法， 由于离子阱装置“缓冲”了API 源和TOF-MS设备，既保持灵敏度，又增强 质谱优值。 在这项研究的第一阶段，IT-TOF被证明具有 许多物理性质和几个分析性质使其成为 一种质量分析工具除了具有非常高的灵敏度（通过 凭借其使用射频在自由空间中“存储”离子的能力， 波），该设备还保留了其作为TOF的大部分特征 仪器，允许在无限质量范围内进行检测， 准实时获取完整质谱。 这项研究的第二阶段将侧重于改进 技术作为一种分析方法，专注于系统 性能增强。将建造一个原型仪器， 演示需要非常高灵敏度的应用， 非常高的扫描速度与液相色谱法结合 或毛细管电泳。 建议的商业应用：主题装置将是“第二个”。 用于高速分离的”飞行时间质谱接口 使用电喷雾和其他大气压电离的系统 技术.它将联合收割机的超高灵敏度， 应用，以及高分辨率和高质量精度， 需要生物分析