RAPID ENZYME AND RECEPTOR ASSAYS USING BIOSOMES

使用生物体进行快速酶和受体测定

• 批准号: 2869890
• 负责人: CHARLES F SALLER
• 金额: $ 40.08万
• 依托单位: ANALYTICAL BIOLOGICAL SERVICES, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2001-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2869890
• 关键词: bioassay; biosensor device; biotechnology; enzyme substrate; fluorescent dye /probe; ligands; liposomes; reagent /indicator; receptor binding; technology /technique development

DESCRIPTION (Verbatim from the applicant's abstract): This research will develop a new molecular recognition technology for drug discovery. Stable liposome polymers, called biosomes, act as biosensors that turn from blue to red in response to a specific molecular recognition event. Biosomes can be functionalized with either an enzyme substrate or receptor ligand so that they respond to a specific receptor or enzyme. A prototypic enzyme assay has been developed using phospholipases. Biosomes also change color in response to interactions with receptors. Phase II research will extend these studies to develop a variety of enzyme assays. The binding studies will be continued with the aim of developing rapid homogenous receptor binding assays. In addition, efforts will be made to greatly increase the sensitivity of Biosome assays by incorporating fluorescent labels into the Biosome Polymers. The Biosome color transition, in response to molecular recognition, will be used to alter fluorescence by means of changes in color quenching or fluorescence energy transfer. The Biosomes should have many applications in high-throughput screening of compounds for a wide range of therapeutic areas. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述（逐字摘自申请人的摘要）：这项研究将 开发用于药物发现的新分子识别技术。稳定的 脂质体聚合物，称为生物体，充当生物传感器，从蓝色转变为 红色响应特定的分子识别事件。生物体可以是 用酶底物或受体配体进行功能化，以便它们 对特定受体或酶作出反应。原型酶测定已 使用磷脂酶开发。生物体也会改变颜色以响应 与受体的相互作用。第二阶段研究将把这些研究扩展到 开发多种酶检测方法。结合研究将继续进行 开发快速同质受体结合测定的目的。此外， 将努力通过以下方式大大提高生物体测定的灵敏度 将荧光标记纳入生物体聚合物中。生物体颜色 响应分子识别的转变将用于改变 通过颜色猝灭或荧光能量的变化产生荧光 转移。生物体应该在高通量方面有很多应用 筛选用于广泛治疗领域的化合物。 拟议的商业应用：不可用