RECOVERY OF FETAL CELLS IN MATERNAL BLOOD

母血中胎儿细胞的回收

基本信息

  • 批准号:
    2889159
  • 负责人:
  • 金额:
    $ 37.94万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1996
  • 资助国家:
    美国
  • 起止时间:
    1996-05-20 至 2000-03-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (Adapted from applicant's abstract): Fetal cells that enter the mother's blood stream during pregnancy can be recovered by charge flow separation (CFS), a method that obviates the risks associated with amniocentesis and chorionic villus sampling. The long-term objective of this project is development of a commercial system for prenatal screening and diagnosis based on recovery by CFS of fetal nucleated red blood cells (NRBC). The aims of the project are: (I) to increase further the speed and efficiency of CFS, and to decrease disposable costs: (II) to test CFS in the screening of common aneuploidies such as trisomy 13, 18, and 21, and to evaluate the specificity and sensitivity of the screening method; (III) to ascertain the life span of fetal NRBC in the maternal circulation; (IV) to culture CFS-processed fetal NRBC, and to discriminate between female fetal NRBC and maternal NRBC after recovery. For analysis of results, the processed fractions will be tested by FISH and primed in situ labeling (PRINS) for sex- chromosome-complement and the common aneuploidies; the results will be compared with those obtained after conventional cytogenetic analysis. Cultures will be evaluated for paternal DNA sequences and for karyotype. With further development, CFS could enable early diagnosis of a broad spectrum of congenital abnormalities, without risk to fetus or mother. Because the different cell types exhibit characteristic surface charge densities and distinctive mobilities in moving fluids, results with CFS are consistent and reproducible, facilitating experimental design and interpretation. Pretreatment of maternal blood samples with antibody is unnecessary. The method is rapid. The recovered cells are viable and the numbers of fetal NRBC obtained are substantially greater than the numbers obtained after separation by other methods. PROPOSED COMMERCIAL APPLICATION: Not available.
描述(改编自申请人摘要):进入 通过充电可以恢复母亲怀孕期间的血流 流动分离(CFS),一种避免与 羊膜穿刺术和绒毛取样。 长期目标 该项目的一个目标是开发一个产前商业系统 基于胎核红CFS恢复的筛查和诊断 血细胞(NRBC)。 该项目的目标是:(一)增加 进一步提高CFS的速度和效率, 成本:(二)测试CFS在筛选常见的非整倍体,如 13、18和21三体,并评估特异性, 筛选方法的敏感性;(三)确定寿命 胎儿NRBC在母体循环;(IV)培养CFS处理 胎儿NRBC,并区分女性胎儿NRBC和母体NRBC 恢复后的NRBC。 为了分析结果,处理的级分 将通过FISH和引物原位标记(PRINS)进行性别检测- 染色体互补和常见的非整倍性;结果将是 与常规细胞遗传学分析后获得的结果相比。 将评价培养物的父系DNA序列和核型。 随着进一步的发展,CFS可以早期诊断广泛的 一系列先天性异常,对胎儿或母亲无风险。 因为不同的细胞类型表现出特征性的表面电荷 密度和独特的流动性在运动的流体,结果与CFS 是一致的和可重复的,有利于实验设计和 解释。 用抗体预处理母体血液样品 没有必要 方法快速。 回收的细胞是活的 并且获得的胎儿NRBC的数量显著大于 用其他方法分离后得到的数字。 拟议的商业应用:不可用。

项目成果

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DAVID W SAMMONS其他文献

DAVID W SAMMONS的其他文献

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{{ truncateString('DAVID W SAMMONS', 18)}}的其他基金

RECOVERY OF FETAL CELLS IN MATERNAL BLOOD
母血中胎儿细胞的回收
  • 批准号:
    2645473
  • 财政年份:
    1996
  • 资助金额:
    $ 37.94万
  • 项目类别:
RECOVERY OF FETAL CELLS IN MATERNAL BLOOD
母血中胎儿细胞的回收
  • 批准号:
    2205920
  • 财政年份:
    1996
  • 资助金额:
    $ 37.94万
  • 项目类别:

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