MABS SPECIFIC TO SEROTONIN 5-HT RECEPTORS

5-羟色胺（5-hydroxytryptamine; 5-HT）介导许多中枢神经系统和 通过与特定神经元的相互作用， 受体。5-HT受体参与各种正常的 生理反应，例如，平滑肌收缩，血小板 聚集、醛固酮产生、昼夜节律和神经元 发展和各种疾病如偏头痛，焦虑， 精神抑郁和不良行为5-HT受体也是 致幻药物的结合点至少16种血清素 已经鉴定了属于七种亚型的受体。更好的 了解定位（作用部位）和监管 5-HT受体的研究可能导致受体特异性药物的开发。 通过SBIR第一阶段的资助，我们开发了单克隆抗体， 对5-HT 2受体的三个成员具有高度特异性 家人作为该项目的延续，我们建议生成 5-HT受体的单克隆抗体。抗体将 筛选它们在免疫组织化学、蛋白质印迹和 免疫沉淀。我们还建议开发分子探针， 通过PCR和RNA酶保护检测和定量5-HT受体 比色法单克隆抗体与PCR探针的组合 和RNase保护试验将为 基础和应用血清素受体研究。 拟议的商业应用： 这项研究的结果将提供工具，可以很容易地 作为研究试剂商业化。目前， 5-HTRs（我们开发的5-HT 2受体除外）和RNase 保护测定探针不可用。自从血清素领域 研究领域很大，超过27，000篇具有潜力的文章 临床应用的意义已发表在过去十年 在未来几年内，我们预计市场将有一个合理的规模。

Serotonin (5-hydroxytryptamine; 5-HT) mediates many central and peripheral nervous functions by its interaction with specific neuronal receptors. The 5-HT receptors are implicated in various normal physiological responses, e.g., smooth muscle contraction, platelet aggregation, aldosterone production, circadian rhythm, and neuronal development and various disorders such as migraine headaches, anxiety, mental depression, and undesired behavior. 5-HT receptors are also binding sites for hallucinogenic drugs. At least sixteen serotonin receptors belonging to seven subtypes have been identified. A better understanding of the localization (the sites of action) and regulation of 5-HT receptors may lead to development of receptor specific drugs. Through SBIR Phase I funding, we have developed monoclonal antibodies that are highly specific to the three members of the 5-HT2 receptor family. As a continuation of this project, we proposed to generate monoclonal antibodies to the rest of the 5-HT receptors. Antibodies will be screened for their utility in immunohistochemistry, western blot, and immunoprecipitation. We also propose to develop molecular probes for detection and quantitation of 5-HT receptors by PCR and RNase protection assay. The combination of monoclonal antibodies and the probes for PCR and RNase protection assay will provide powerful research tools for fundamental and applied serotonin receptor research. PROPOSED COMMERCIAL APPLICATIONS: The result of this study will provide tools, which can be readily commercialized as research reagents. At present monoclonal antibodies to 5-HTRs (other than to 5-HT2 receptor developed by us) and RNase protection assay probes are not available. Since the field of serotonin research field is large and more than 27,000 articles with potential significance for clinical application have been published in the last ten years, we predict the market to be of a reasonable size.