MABS SPECIFIC TO METABOTROPIC GLUTAMATE RECEPTORS

描述(摘自申请者的摘要)：谷氨酸是 哺乳动物大脑中主要的快速兴奋性神经递质和 通过与特异体的相互作用来调节许多神经系统功能 神经元受体。生理学、药理学和分子生物学 克隆研究已经确定了两种主要的谷氨酸 受体，称为离子亲和性(GluR)和代谢性(MGluR)。这些 受体在学习和记忆、神经元中都很重要。 在神经退行性疾病的发病机制中起重要作用。这个 MGluR的分子克隆为更好地了解 这些药理上重要的受体的结构和功能。 详细了解谷氨酸的解剖结构和亚型特异性 受体在理解正常生理和 疾病中发生的病理变化。更好地理解 MGluRs的本地化和调节可能导致 受体特异性药物。单抗(MAbbs)不是 可用于不同的mGluR亚型。利用多肽和重组 将蛋白质作为免疫原单抗制成mGluR亚型。抗体 将对其在免疫组织化学和Western中的效用进行筛选 污点。申请人声称，抗mGluRs的单抗 将是免疫定位和调节研究的有价值的工具。

DESCRIPTION (adapted from applicant's abstract): Glutamate is the major fast excitatory neurotransmitter in the mammalian brain and mediates many nervous system functions by its interaction with specific neuronal receptors. Physiological, pharmacological, and molecular cloning studies have identified two major classes of glutamate receptors, called ionotropic(GluR) and metabotropic (mGluR). These receptors are important in learning and memory, in neuronal development, and in the pathogenesis of neurodegenerative diseases. The molecular cloning of mGluR provides a great understanding of the structure and function of these pharmacologically important receptors. Detailed knowledge of the anatomy and subtype specificity of glutamate receptors is important both in understanding the normal physiology and the pathological changes occurring in diseases. A better understanding of the localization and regulation of mGluRs may lead to development of receptor specific drugs. Monoclonal antibodies (mAbs) are not available to different mGluR subtypes. Using peptides and recombinant proteins as immunogens mAbs will be made to mGluR subtypes. Antibodies will be screened for their utility in immunohistochemistry and Western blot. The applicant claims that monoclonal antibodies to the mGluRs will be valuable tools for immunolocalization and regulation studies.