RECEPTOR--TYROSINE PHOSPHATASE LAR IN LUNG DEVELOPMENT
RECEPTOR--TYROSINE PHOSPHATASE LAR IN LUNG DEVELOPMENT
批准号:
2857849
负责人:
QIANG YU
金额:
$33.39万
依托单位国家:
美国
项目类别:
财政年份:
1996
资助国家:
美国
项目状态:
已结题
起止时间:
1996-01-01 至 2000-07-31
关键词:
affinity chromatography biological signal transduction cell cycle cell differentiation cell growth regulation embryo /fetus tissue /cell culture flow cytometry growth /development growth factor receptors histocompatibility antigens laboratory rat lung northern blottings protein tyrosine phosphatase receptor respiratory epithelium tissue /cell culture
中文摘要
描述(改编自申请者的抽象和具体目标):
这个项目的目标是定义特定受体的作用
酪氨酸磷酸酶(PTPase)在调节上皮细胞中的作用
发育和成熟肺的增殖和分化。
酪氨酸PTPase使具有以下特征的蛋白质上的磷酸酪氨酸去磷酸化
被特定的酪氨酸激活或失活(磷酸化)
其中许多是生长因子受体。因此,PTPase具有
调节相关的各种细胞信号事件的可能性
与发育和细胞分化有关。申请的前提是
跨膜酪氨酸磷酸酶LAR可能负向调节
与生长因子受体相互作用的细胞生长
固有的酪氨酸激酶活性,这一功能是
在肺上皮细胞中很重要,特别是在肺发育过程中。
具体目标是:1)开发方法和试剂以改变
LAR的生物学活性及其对上皮细胞的影响
胎肺器官培养体系中的增殖和分化;
2)确定LAR在调节细胞增殖中的潜在作用
以及调节生长因子介导的信号转导;3)
通过识别和识别确定LAR功能的具体机制
研究LAR与之相互作用的蛋白质。
英文摘要
DESCRIPTION (Adapted from the applicant's abstract and specific aims):
The goal of this project is to define the role that a specific receptor
type tyrosine phosphatase (PTPase) plays in regulating epithelial cell
proliferation and differentiation in developing and mature lung.
Tyrosine PTPases dephosphorylate phospho-tyrosines on proteins that have
been activated or inactivated (phosphorylated) by specific tyrosine
kinases of which many are growth factor receptors. Thus, PTPases have
the potential to regulate a variety of cell signaling events associated
with development and cell differentiation. The application postulates
that the transmembrane tyrosine phosphatase LAR may negatively regulate
cell growth by interacting with growth factor receptors possessing
intrinsic tyrosine kinase activities, and that this function is
important in lung epithelial cells, especially during lung development.
The specific aims are to: 1) develop methods and reagents to alter
biological activities of LAR and define their effects on epithelial cell
proliferation and differentiation in a fetal lung organ culture system;
2) determine the potential roles of LAR in regulating cell proliferation
and in modulating growth factor-mediated signal transduction; and 3)
determine specific mechanism of LAR function by identifying and
characterizing proteins with which LAR interacts.
期刊论文(4)
专著(0)
科研奖励(0)
会议论文
Specific inhibition of FGF-induced MAPK activation by the receptor-like protein tyrosine phosphatase LAR.
受体样蛋白酪氨酸磷酸酶 LAR 特异性抑制 FGF 诱导的 MAPK 激活。
DOI:
10.1038/sj.onc.1203558
发表时间:
2000
期刊:
Oncogene
影响因子:
8
作者:
[Wang,X, Weng,LP, Yu,Q]
通讯作者:
Yu,Q
Transmembrane tyrosine phosphatase LAR induces apoptosis by dephosphorylating and destabilizing p130Cas.
跨膜酪氨酸磷酸酶 LAR 通过使 p130Cas 去磷酸化和不稳定来诱导细胞凋亡。
DOI:
10.1046/j.1365-2443.1999.00251.x
发表时间:
1999
期刊:
Genes to cells : devoted to molecular & cellular mechanisms
影响因子:
--
作者:
[Weng,LP, Wang,X, Yu,Q]
通讯作者:
Yu,Q
Overexpression of the transmembrane tyrosine phosphatase LAR activates the caspase pathway and induces apoptosis.
跨膜酪氨酸磷酸酶 LAR 的过度表达会激活 caspase 途径并诱导细胞凋亡。
DOI:
10.1016/s0960-9822(98)70106-x
发表时间:
1998
期刊:
Current biology : CB
影响因子:
--
作者:
[Weng,LP, Yuan,J, Yu,Q]
通讯作者:
Yu,Q
CORE--MOLECULAR BIOLOGY
-
批准号:6501914
-
项目类别:
-
资助金额:$8.86万
-
财政年份:2001
-
负责人:QIANG YU
-
依托单位:
CORE--MOLECULAR BIOLOGY
-
批准号:6324749
-
项目类别:
-
资助金额:$33.03万
-
财政年份:2000
-
负责人:QIANG YU
-
依托单位:
FUNCTION OF P130 CAS IN CELL SURVIVAL
-
批准号:6526055
-
项目类别:
-
资助金额:$27.15万
-
财政年份:1999
-
负责人:QIANG YU
-
依托单位:
FUNCTION OF P130 CAS IN CELL SURVIVAL
-
批准号:6182024
-
项目类别:
-
资助金额:$25.61万
-
财政年份:1999
-
负责人:QIANG YU
-
依托单位:
FUNCTION OF P130 CAS IN CELL SURVIVAL
-
批准号:6386530
-
项目类别:
-
资助金额:$26.37万
-
财政年份:1999
-
负责人:QIANG YU
-
依托单位:
CORE--MOLECULAR BIOLOGY
-
批准号:6110106
-
项目类别:
-
资助金额:$33.03万
-
财政年份:1999
-
负责人:QIANG YU
-
依托单位:
FUNCTION OF P130 CAS IN CELL SURVIVAL
-
批准号:2883932
-
项目类别:
-
资助金额:$24.87万
-
财政年份:1999
-
负责人:QIANG YU
-
依托单位:
CORE--MOLECULAR BIOLOGY
-
批准号:6272906
-
项目类别:
-
资助金额:$32.4万
-
财政年份:1998
-
负责人:QIANG YU
-
依托单位:
CORE--MOLECULAR BIOLOGY
-
批准号:6242153
-
项目类别:
-
资助金额:$31.65万
-
财政年份:1997
-
负责人:QIANG YU
-
依托单位:
RECEPTOR--TYROSINE PHOSPHATASE LAR IN LUNG DEVELOPMENT
-
批准号:2029351
-
项目类别:
-
资助金额:$31.73万
-
财政年份:1996
-
负责人:QIANG YU
-
依托单位:
RECEPTOR--TYROSINE PHOSPHATASE LAR IN LUNG DEVELOPMENT
-
批准号:1073100
-
项目类别:
-
资助金额:$4.19万
-
财政年份:1996
-
负责人:QIANG YU
-
依托单位:
RECEPTOR--TYROSINE PHOSPHATASE LAR IN LUNG DEVELOPMENT
-
批准号:2638043
-
项目类别:
-
资助金额:$33.05万
-
财政年份:1996
-
负责人:QIANG YU
-
依托单位:
RECEPTOR--TYROSINE PHOSPHATASE LAR IN LUNG DEVELOPMENT
-
批准号:2232237
-
项目类别:
-
资助金额:$23.44万
-
财政年份:1996
-
负责人:QIANG YU
-
依托单位:
CORE--MOLECULAR BIOLOGY
-
批准号:5213915
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
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负责人:QIANG YU
-
依托单位:--
CORE--MOLECULAR BIOLOGY
-
批准号:3736964
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项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:QIANG YU
-
依托单位:
CORE--MOLECULAR BIOLOGY
-
批准号:3758983
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:QIANG YU
-
依托单位:
海外基金