HISTATIN RECEPTORS AS DRUG TARGETS FOR ORAL CANDIDIASIS

组氨酸受体作为口腔念珠菌病的药物靶点

• 批准号: 2449485
• 负责人: Mira Edgerton
• 金额: $ 8.36万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2004-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2449485
• 关键词: Candida albicans; HIV infections; antiAIDS agent; antifungal agents; binding proteins; candidiasis; complementary DNA; computer assisted sequence analysis; drug resistance; fungal genetics; fungal proteins; gene expression; genetic library; human tissue; laboratory rabbit; mass spectrometry; membrane potentials; membrane transport proteins; nucleic acid sequence; opportunistic infections; pathologic process; polymerase chain reaction; protein structure function; saliva; virulence

This is a new application for an Independent Scientist Award (K02) for Dr. Mira Edgerton, and it accompanies the already funded R01DE12159 grant. Dr. Edgerton has a D.D.S. degree, specialty training in prosthodontics, and a Ph.D. in Oral Biology. She is an Assistant Professor in the Departments of Restorative Dentistry and Oral Biology at the State University of New York at Buffalo (SUNYAB). Dr. Edgerton's research has focused on characterizing the structural elements of salivary histatins required for candidacidal activity as well as the cellular mechanism of action of histatins with Candida albicans. Her work has identified a C. albicans membrane protein (HstBP) which is a yeast receptor protein of the histatin candidacidal pathway. The current research project will clone and sequence the C. albicans HstBP gene and examine its expression as a virulence or resistance factor in yeast from HIV-oropharyngeal candidiasis patients. Dr. Edgerton's immediate goals are to use the most current techniques in yeast molecular genetic to identify mechanisms of C. albicans pathogenicity in the oral cavity. Her long range goals are to define the molecular basis of virulence, pathogenesis and drug resistance of C. albicans in order to develop better treatment modalities for local and systemic candidiasis. Achievement of these goals is possible only through acquisition of an in-depth background in yeast molecular genetics and knowledge of the most current systems fir eukaryotic genetics. Dr. Edgerton's scientific career would be advanced through this additional training and experience. The research environment in the Department of Oral Biology at SUNYAB in combination with experiences in the laboratories of other yeast molecular geneticists is excellent for development of the research career of Dr. Edgerton. However, her clinical teaching duties limit the time available to her for acquiring these additional research skills. This ISA application is to obtain salary support to release Dr. Edgerton from her clinical teaching and committee responsibilities in order to devote 80% of her full time professional effort to research activities. This award would provide her the opportunity to extend her background in yeast biology to the molecular level and apply this new expertise to studies of virulence and pathogenesis of oral and systemic candidiasis.

这是米拉·埃格顿（Mira Edgerton）博士的独立科学家奖（K02）的新申请，并伴随已有资助的R01DE12159赠款。 Edgerton博士有D.D.S.学位，假牙专业培训和博士学位。在口腔生物学中。她是纽约州立大学（Sunyab）恢复性牙科和口腔生物学系的助理教授。 Edgerton博士的研究重点是表征念珠菌活性所需的唾液组蛋白的结构元素，以及与白色念珠菌念珠菌的组织素作用机理。她的工作已经确定了白色念珠菌膜蛋白（HSTBP），该蛋白是组织蛋白念珠菌途径的酵母受体蛋白。当前的研究项目将克隆并对白色念珠菌HSTBP基因进行序列，并研究其表达是HIV-咽喉念珠菌患者的酵母中的毒力或抗性因子。 Edgerton博士的近期目标是在酵母分子遗传中使用最新技术来识别口腔中白色念珠菌致病性的机制。她的远距离目标是定义白色念珠菌的毒力，发病机理和耐药性的分子基础，以开发针对局部和全身性念珠菌病的更好的治疗方式。只有通过获取酵母分子遗传学的深入背景以及对最新系统的知识，才能实现这些目标。 Edgerton博士的科学生涯将通过这种额外的培训和经验来提高。 Sunyab口腔生物学系的研究环境与其他酵母分子遗传学家实验室的经验相结合，非常适合发展Edgerton博士的研究生涯。但是，她的临床教学职责限制了她获得这些额外研究技能的时间。 ISA的申请是为了获得薪水支持，以将Edgerton博士从临床教学和委员会的责任中释放出来，以便将她的全职专业努力的80％用于研究活动。该奖项将使她有机会将酵母生物学的背景扩展到分子水平，并将这种新的专业知识应用于口服和系统性念珠菌病的毒力和发病机理的研究。