HISTATIN RECEPTORS AS DRUG TARGETS FOR ORAL CANDIDIASIS

组氨酸受体作为口腔念珠菌病的药物靶点

• 批准号: 2856658
• 负责人: Mira Edgerton
• 金额: $ 10.56万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2856658
• 关键词: Candida albicans; HIV infections; antifungal agents; candidiasis; clinical research; drug receptors; drug screening /evaluation; genetic strain; human subject; molecular cloning; protein purification; protein structure function; receptor binding; receptor expression; saliva

DESCRIPTION: (lifted from Abstract): Histatins (Hst) are one of the major salivary proteins that appear to have fungicidal activity in the mouth. Oropharyngeal candidiasis (OPC) in HIV-infected individuals may be the result of an alteration in the interactions between Hst and Candida albicans. These researchers have identified a surface protein on Candida, HstBP, that may be the receptor or binding protein for Hst. They suggest that HstBP may be related to efflux pumps such as the ABC transporters or the Major Facilitator superfamily. They hypothesize that OPC may be the result of Candida strains with altered expression of HstBP. They propose to characterize HstBP expression as an underlying mechanism in the development of candidiasis in HIV patients by the following specific aims: 1. Purify HstBP by standard biochemical methods, clone and sequence the HstBP gene. 2. Define the biologic role of HstBP by identifying common structural features, and by assessing the effects of certain physiological mediators on HstBP. 3. Compare HstBP expression in yeasts that are sensitive or resistant to Hst from patients with OPC by determining HstBP molecules/cell using standard binding assays and by levels of mRNA expression using Northern blot analysis.

描述：（摘自摘要）：组氨酸 (Hst) 是主要的抑制素之一 唾液蛋白似乎在口腔中具有杀菌活性。 HIV 感染者的口咽念珠菌病 (OPC) 可能是 Hst 和念珠菌之间相互作用改变的结果 白色念珠菌。 这些研究人员已经鉴定出念珠菌的表面蛋白， HstBP，可能是Hst的受体或结合蛋白。他们建议 HstBP 可能与外排泵有关，例如 ABC 转运蛋白或 主要促进者超家族。 他们推测 OPC 可能是 HstBP 表达改变的念珠菌菌株的结果。 他们提议 将 HstBP 表达描述为发育的潜在机制 通过以下具体目标来控制 HIV 患者中的念珠菌病： 1. 净化 HstBP通过标准生化方法，对HstBP基因进行克隆和测序。 2. 通过识别共同结构来定义 HstBP 的生物学作用 特征，并通过评估某些生理介质的影响 热血压。 3. 比较敏感或敏感酵母中的 HstBP 表达 通过确定 HstBP 分子/细胞来确定 OPC 患者对 Hst 的抵抗力 使用标准结合测定并通过 mRNA 表达水平 Northern印迹分析。