HISTATIN RECEPTORS AS DRUG TARGETS FOR ORAL CANDIDIASIS

组氨酸受体作为口腔念珠菌病的药物靶点

• 批准号: 2856658
• 负责人: Mira Edgerton
• 金额: $ 10.56万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2856658
• 关键词: Candida albicans; HIV infections; antifungal agents; candidiasis; clinical research; drug receptors; drug screening /evaluation; genetic strain; human subject; molecular cloning; protein purification; protein structure function; receptor binding; receptor expression; saliva

DESCRIPTION: (lifted from Abstract): Histatins (Hst) are one of the major salivary proteins that appear to have fungicidal activity in the mouth. Oropharyngeal candidiasis (OPC) in HIV-infected individuals may be the result of an alteration in the interactions between Hst and Candida albicans. These researchers have identified a surface protein on Candida, HstBP, that may be the receptor or binding protein for Hst. They suggest that HstBP may be related to efflux pumps such as the ABC transporters or the Major Facilitator superfamily. They hypothesize that OPC may be the result of Candida strains with altered expression of HstBP. They propose to characterize HstBP expression as an underlying mechanism in the development of candidiasis in HIV patients by the following specific aims: 1. Purify HstBP by standard biochemical methods, clone and sequence the HstBP gene. 2. Define the biologic role of HstBP by identifying common structural features, and by assessing the effects of certain physiological mediators on HstBP. 3. Compare HstBP expression in yeasts that are sensitive or resistant to Hst from patients with OPC by determining HstBP molecules/cell using standard binding assays and by levels of mRNA expression using Northern blot analysis.

描述：(摘自摘要)：组蛋白(HST)是主要的 唾液蛋白在口腔中似乎具有杀菌活性。 口咽念珠菌病(OPC)在HIV感染者中可能是 HST与念珠菌相互作用改变的结果 白念珠菌。这些研究人员已经确定了念珠菌上的一种表面蛋白， HstBP，可能是HST的受体或结合蛋白。他们建议 HstBP可能与外排泵有关，如ABC转运体或 主要促进者超级家族。他们假设OPC可能是 HstBP表达改变的念珠菌的检测结果。他们提议 HstBP的表达是发育中的一种潜在机制 通过以下特定目的控制HIV患者中的念珠菌病：1.纯化 采用标准生化方法，对HstBP基因进行克隆和测序。 2.通过鉴定共同的结构确定HstBP的生物学作用 特征，并通过评估某些生理介质对 HstBP。3.比较HstBP在敏感或敏感酵母中的表达 检测HstBP分子/细胞检测OPC患者对HST的耐药性 使用标准结合分析和使用 Northern印迹分析。