PROFILING OF PLASMA APOLIPOPROTEINS BY HPCE AND MS

通过 HPCE 和 MS 分析血浆载脂蛋白

• 批准号: 2750474
• 负责人: RONALD D MACFARLANE
• 金额: $ 22.12万
• 依托单位: TEXAS A&M UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2750474
• 关键词: apolipoproteins; atherosclerosis; blood chemistry; capillary electrophoresis; electrospray ionization mass spectrometry; high density lipoproteins; high performance liquid chromatography; low density lipoprotein; method development; protein isoforms; triglycerides

DESCRIPTION: The hypothesis of this proposal is that a lipid screen based solely on the measurement of HDL-cholesterol, LDL- cholesterol and triglyceride levels is so restricted that a significant fraction of individuals at risk for cardiovascular diseases are not identified. Inclusion of the major apolipoproteins in the clinical screening protocol as an option would identify a greater fraction of these individuals. The specific aim of this project is to develop new chemical methodology for accurate measurements of plasma levels of apolipoproteins and to identify their isoform distributions. The goal is to develop a separations and quantitation analysis scheme for apolipoproteins based on capillary electrophoresis and electrospray ionization mass spectrometry. The long term objective is to more efficiently identify individuals at risk for the development of cardiovascular disease through measurement and interpretation of lipoprotein particle density profiles and to strengthen the analytical base to provide a better understanding of the role of apolipoproteins in atherogenesis.

描述：该提议的假设是基于脂质屏幕 仅根据测量HDL-胆固醇，LDL-胆固醇和 甘油三酸酯水平受到限制，以至于很大一部分 未发现有心血管疾病风险的人。 将主要的载脂蛋白包括在临床筛查方案中 一种选择将确定这些人的较大部分。 这 该项目的具体目的是开发新的化学方法论 准确测量载脂蛋白的血浆水平，并确定 他们的同工型分布。 目标是建立分离和 基于毛细管的载脂蛋白的定量分析方案 电泳和电喷雾电离质谱法。 长 术语目标是更有效地识别有风险的个人 通过测量和解释发展心血管疾病 脂蛋白颗粒密度曲线并增强分析 基础以更好地理解载脂蛋白在 动脉粥样硬化。