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CELL-SPECIFIC EXPRESSION IN THE PITUITARY GLAND

CELL-SPECIFIC EXPRESSION IN THE PITUITARY GLAND
垂体中的细胞特异性表达
批准号：
6182580
负责人：
Sally A. Camper
金额：
$28.99万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
项目状态：
已结题
起止时间：
1999-07-15 至 2004-06-30
来源：
https://reporter.nih.gov/project-details/6182580
关键词：
RNase protection assay cell line developmental genetics gene expression gene targeting genetically modified animals histogenesis immunocytochemistry in situ hybridization laboratory mouse pituitary gland protein isoforms transcription factor transfection

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英文摘要
DESCRIPTION: (Adapted from the applicant's abstract) The investigator will generate and analyze hypomorphic, null and cell-specific gene modifications of Pitx2. Specific antibodies will be generated to assess the cell specificity of Pitx2 expression in the pituitary and other organs of intact animals. Several pituitary hormone genes have binding sites for Pitx2. There are three isoforms of Pitx2, two of which are expressed in the pituitary gland (Pitx2a, Pitx2b). The relative activity of each Pitx2 isoform on expression of known target genes will be assessed by transient transfection studies using homologous pituitary cell lines. The effects of ectopic Pitx2a and Pitx2b expression in pituitary corticotropes will also be investigated using both transient transfection and transgenic mice. By combining the approaches of transfection, embryonic stem cell technology mice, the investigator will dissect the contributions of individual cells and individual genes in regulating the normal growth and development of the pituitary.
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