CELL-SPECIFIC EXPRESSION IN THE PITUITARY GLAND

描述：（改编自申请人摘要）研究者将 生成和分析亚纯型、无效和细胞特异性基因修饰， Pitx2。将产生特异性抗体以评估细胞特异性。 Pitx2在完整动物垂体和其他器官中的表达。几 垂体激素基因具有Pitx2的结合位点。有三种亚型 Pitx2的基因，其中两个在脑垂体中表达（Pitx2a，Pitx2b）。 每种Pitx2同种型对已知靶基因表达的相对活性 将通过使用同源垂体的瞬时转染研究来评估 细胞系垂体异位Pitx2a和Pitx2b表达的影响 还将使用瞬时转染和 转基因小鼠结合转染方法， 细胞技术小鼠，研究人员将剖析的贡献， 单个细胞和单个基因调节正常生长， 垂体的发育。

DESCRIPTION: (Adapted from the applicant's abstract) The investigator will generate and analyze hypomorphic, null and cell-specific gene modifications of Pitx2. Specific antibodies will be generated to assess the cell specificity of Pitx2 expression in the pituitary and other organs of intact animals. Several pituitary hormone genes have binding sites for Pitx2. There are three isoforms of Pitx2, two of which are expressed in the pituitary gland (Pitx2a, Pitx2b). The relative activity of each Pitx2 isoform on expression of known target genes will be assessed by transient transfection studies using homologous pituitary cell lines. The effects of ectopic Pitx2a and Pitx2b expression in pituitary corticotropes will also be investigated using both transient transfection and transgenic mice. By combining the approaches of transfection, embryonic stem cell technology mice, the investigator will dissect the contributions of individual cells and individual genes in regulating the normal growth and development of the pituitary.