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Exploration of the oogenic potential of putative germ line stem cells isolated from the ovaries of girls and adult women

探索从女孩和成年女性卵巢中分离的假定生殖系干细胞的卵子潜能

基本信息

批准号：
MR/R003246/1
负责人：
Evelyn Telfer
金额：
$ 98.06万
依托单位：
University of Edinburgh
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2017
资助国家：
英国
起止时间：
2017 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=MR%2FR003246%2F1
关键词：
Exploration oogenic potential putative germ

项目摘要

For decades, scientists have believed that women were born with all the eggs they would ever have and that no new ones could ever be made. Recent work has shown that the ovaries of adult women have cells that are capable of forming what appears to be new eggs under certain conditions. The implications of this are potentially enormous. If we could make new eggs we could eventually help restore fertility in women who have become infertile for a variety of reasons, including chemotherapy treatment, early menopause or just normal aging. We need to learn more about these cells and determine the conditions that allow them to form eggs and to know whether the eggs are normal. We have recently isolated two populations of cells and we need to study them in more detail. This project will define the cells that can be isolated from ovarian material from a range of ages. Then using a system where we can grow eggs outside the body (culture system), we will determine whether these cells can form new eggs and whether they are normal. We will also find out the conditions that support this process, The ultimate end point of this work would be to determine whether these cells were capable of forming eggs that could be fertilised and form normal embryos.

几十年来，科学家们一直认为，女性出生时就已经拥有了所有的卵子，而且永远不会产生新的卵子。最近的研究表明，成年女性的卵巢细胞在某些条件下能够形成新的卵子。这一点的影响可能是巨大的。如果我们能够制造新的卵子，我们最终可以帮助那些因各种原因而不孕的女性恢复生育能力，包括化疗治疗，更年期提前或正常衰老。我们需要更多地了解这些细胞，并确定允许它们形成卵子的条件，并了解卵子是否正常。我们最近分离了两个细胞群，我们需要更详细地研究它们。该项目将定义可以从不同年龄的卵巢材料中分离的细胞。然后使用我们可以在体外培养卵子的系统（培养系统），我们将确定这些细胞是否可以形成新的卵子，以及它们是否正常。我们还将找出支持这一过程的条件，这项工作的最终目的是确定这些细胞是否能够形成可以受精并形成正常胚胎的卵子。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Workflow Optimization for Identification of Female Germline or Oogonial Stem Cells in Human Ovarian Cortex Using Single-Cell RNA Sequence Analysis.

DOI：
10.1093/stmcls/sxac015
发表时间：
2022-05-27
期刊：
Stem cells (Dayton, Ohio)
影响因子：
0
作者：
通讯作者：

Spatio-temporal remodelling of the composition and architecture of the human ovarian cortical extracellular matrix during in vitro culture.

DOI：
10.1093/humrep/dead008
发表时间：
2023-03-01
期刊：
Human reproduction (Oxford, England)
影响因子：
0
作者：
通讯作者：

Initial characterisation of adult human ovarian cell populations isolated by DDX4 expression and aldehyde dehydrogenase activity.

DOI：
10.1038/s41598-018-25116-1
发表时间：
2018-05-03
期刊：
Scientific reports
影响因子：
4.6
作者：
Clarkson YL;McLaughlin M;Waterfall M;Dunlop CE;Skehel PA;Anderson RA;Telfer EE
通讯作者：
Telfer EE

Being a good egg in the 21st century.

在21世纪成为一个好鸡蛋。