ELECTROCHEMICAL BIOSENSOR OF CARDIAC MARKERS

心脏标记物电化学生物传感器

• 批准号: 6072572
• 负责人: KAI H WANG
• 金额: $ 12.03万
• 依托单位: INTEC SCIENCE, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-22 至 2001-03-21
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6072572
• 关键词: biomarker; biomedical equipment development; biosensor device; diagnosis design /evaluation; electrochemistry; heart disorder diagnosis; immunoaffinity chromatography; myocardial infarction; myoglobin; troponin

Development of a novel immunochromatographic electrochemical biosensor(IEB) is proposed for simultaneous quantitation of Troponin-I, Myoglobin and CK-MB, which have been reported to be important indexes in the diagnosis of myocardial infraction. Such biosensor using electrochemical detectors will avoid optical interference in the current immunoassays, and will provide more accuracy and sensitivity to rapidly quantify analytes. Our preliminary results using the proposed methodology for quantitation of myoglobin demonstrated several advantages over the current existed method such as high sensitivity, reproducibility, rapidity and convenience in use. We propose to further optimize this novel method for simultaneous quantitation of the above mentioned cardiac bio-markers, and develop a portable biosensor in point-of-care application. The proposed biosensor will consist of an immunochromatographic strip which produces immuno-complexes of these cardiac markers with their specific antibodies labeled with different metal ions and an electrochemical detector which quantifies the amounts of these metal ions in the complex. Design of the IEB will be carefully developed, and simultaneous quantitation of metal ions by the electrode in the test strips will also be evaluated and optimized. Such biosensor will provide essential information for proper clinical decision, and thus greatly improve the quality of coronary care. PROPOSED COMMERCIAL APPLICATIONS: About 16 million cardiac examinations were conducted annually in the United States. A method with rapidity, high specificity and sensitivity, clinical accuracy is urgent to be developed for simultaneous quantitation of the cardiac markers in coronary care. The proposed immunochromatographic electrochemical biosensor is expected to provide these characteristics of myocardial infarction diagnosis at emergency room or coronary care units. Such biosensor will provide a low cost, highly efficient, clinically accurate and simple operation without technical training, and thus will have additional potential in commercial applications.

提出了一种新型的免疫层析电化学生物传感器（IEB），用于同时定量肌钙蛋白- i、肌红蛋白和CK-MB，这是诊断心肌梗死的重要指标。这种使用电化学检测器的生物传感器将避免当前免疫分析中的光学干扰，并将为快速定量分析提供更高的准确性和灵敏度。我们使用所提出的方法定量肌红蛋白的初步结果表明，与现有方法相比，该方法具有灵敏度高、重复性好、快速和使用方便等优点。我们建议进一步优化这种同时定量上述心脏生物标志物的新方法，并开发一种便携式生物传感器在护理点应用。提出的生物传感器将由免疫层析条组成，该层析条产生这些心脏标记物的免疫复合物，其特异性抗体被标记为不同的金属离子，以及电化学探测器，该探测器量化复合物中这些金属离子的数量。将仔细开发IEB的设计，并对测试条中电极的金属离子同时定量进行评估和优化。这种生物传感器将为正确的临床决策提供必要的信息，从而大大提高冠状动脉护理的质量。拟议的商业应用：在美国，每年约有1600万例心脏检查。一种快速、高特异性、高灵敏度、临床准确的冠状动脉护理中心脏标志物的同时定量方法是迫切需要的。所提出的免疫层析电化学生物传感器有望在急诊室或冠状动脉护理单位提供心肌梗死诊断的这些特征。这种生物传感器具有成本低、效率高、临床准确、操作简单、无需技术培训等优点，具有较大的商业应用潜力。