Human gastruloids: an in vitro system for the study of human gastrulation

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The discovery that mouse and human Embryonic Stem Cells (ESCs) can be coaxed in vitro to form tissues and organs has raised expectations that the associated protocols for guided differentiation in culture will provide a basis for the successful development of a rational Regenerative Medicine. However it is early days. At the moment the most successful experiments are being done with mouse ESCs because they allow a wide range of experiments in a well understood and standardized system. Also, while there are protocols that can generate organ like structures -called organoids- in vitro, the protocols are neither robust nor reproducible enough to be if much use. A reason for this is that while we have made great advances in our understanding of the genes that control the emergence of specific cell types, we have little understanding of how the expression of these genes is translated into the three dimensional structures that characterize tissues and organs. Understanding this requires a user friendly three dimensional experimental system that mimics the embryo. The situation is even more challenging in the case of human tissues and organs since, in this case, we have very little insight on the development of human embryos and negligible knowledge of their cellular and molecular underpinning. Making progress in this important area of research is also limited by obvious ethical issues and regulations associated with the use of human embryos. ESCs provide an alternative experimental system but current studies with human ESCs restrict themselves to adherent cultures or use the mouse and mouse ESCs as a standard reference for human biology because of its superficial similarities in the underlying developmental processes. However, there are significant differences between the two species that, when considered in detail, highlight the need for a better understanding of the specific cellular and molecular features underlying human embryogenesis. Furthermore, adherent cultures do not reflect the complexities and interactions that emerge in three dimensional structures. Increasingly, other primates are being studied and used as referenced but this does not remove the ethical issues that are associated with this kind of study.Over the last few years we have established an experimental system based on mouse ESCs which, surprisingly, reproduces to a very high degree the early stages of embryonic development and patterning, most notably, the process of gastrulation. The structures mirror the embryo until, approximately, stage 10 (almost half way through its development). An important feature of these organoids, that we call 'gastruloids' because of their ability to imitate gastrulation, is that they develop in the absence of the extraembryonic tissues that mediate the interactions between the embryo and the mother. A second feature is that they lack a head and brain and consist,only, of structures from the neck down. Here we propose to build on these observations and preliminary results that we have obtained to establish a similar system for human ESCs. This will allow an unprecedented access to the mechanisms underlying the early stages of human development and, in particular, the cellular and molecular processes that underlie the establishment of primordial for tissues and organs. Furthermore, the lack of a brain and a head and importantly, the inability of these organoids to interact with the mother account for many of the ethical considerations that apply to embryos.