IMPROVED RADIOIODINATION METHODS FOR RADIOIMMUNOTHERAPY

改进的放射免疫治疗的放射碘定位方法

• 批准号: 6172916
• 负责人: SERENGULAM V GOVINDAN
• 金额: $ 27.55万
• 依托单位: IMMUNOMEDICS, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6172916
• 关键词: breast neoplasms; cell line; enzyme linked immunosorbent assay; immunological substance; immunotherapy; iodination; laboratory mouse; method development; monoclonal antibody; radiation therapy; radiotracer; tumor antigens

The ultimate goal of this work is to produce clinically useful, commercially viable, therapy agent for breast cancer. Radioimmunotherapy has shown clinical efficacy against radiosensitive lymphomas in recent years, and is considered a potentially attractive modality for treating metastatic breast cancer. The therapy agent will be based on a humanized monoclonal antibody (hMAb) hRS7, which targets a breast cancer antigen and which would minimize patient immune response to MAb in eventual clinical application. Phase II work will involve humanization of RS7, process optimizations for clinical-scale radioiodinations, and extensive experimentations to demonstrate preclinical utility of the proposed agent in subcutaneous and systemic animal models of breast cancer. The internalizing hRS7 will be radiolabeled with a residualizing form of I-131 radionuclide, developed in Phase I, so as to increase the retention of the nuclide in the tumor and thereby enhance therapeutic efficacy. The goals are to achieve clinical- scale radiolabelings of hRS7 with >90% efficiency and about 10 mCi/mg of specific activity, and establish significant therapeutic advantage of the optimized product compared to conventionally radioiodinated hRS7, irrelevant hMAb and untreated control. Promising radiochemistry and preclinical results will form the basis for clinical studies in Phase III. PROPOSED COMMERCIAL APPLICATIONS: Radioimmunotherapy (RAIT) is a promising treatment modality, worthy of exploration as adjuvant systemic therapy of metastatic breast cancer in patients with poor prognosis. To realize the full potential of RAIT in this regard, commercially viable optimum delivery agents, such as improved radioiodinated humanized antibodies, must be used. The proposed Phase II research addresses these important issues.

这项工作的最终目标是生产临床上有用的，商业上可行的，乳腺癌的治疗剂。近年来，放射免疫治疗已显示出对放射敏感性淋巴瘤的临床疗效，并被认为是治疗转移性乳腺癌的潜在有吸引力的方式。治疗剂将基于人源化单克隆抗体（hMAb）hRS 7，其靶向乳腺癌抗原并且在最终临床应用中将使患者对MAb的免疫应答最小化。第二阶段的工作将涉及RS7的人源化，临床规模放射性碘化的工艺优化，以及广泛的实验，以证明所提出的药剂在乳腺癌皮下和全身动物模型中的临床前效用。内化hRS 7将用I期开发的I-131放射性核素的残留形式放射性标记，以增加核素在肿瘤中的保留，从而增强治疗功效。目标是以>90%的效率和约10 mCi/mg的比活性实现hRS 7的临床规模放射性标记，并建立优化产物与常规放射性碘标记的hRS 7、不相关的hMAb和未处理的对照相比的显著治疗优势。有前途的放射化学和临床前结果将构成III期临床研究的基础。拟议的商业应用：放射免疫治疗（赖特）是一种很有前途的治疗方式，值得探索作为预后不良的转移性乳腺癌患者的辅助全身治疗。为了实现赖特在这方面的全部潜力，必须使用商业上可行的最佳递送剂，例如改进的放射性碘标记的人源化抗体。拟议的第二阶段研究将解决这些重要问题。