CHEMOKINE SYNERGY WITH PDGF--OLIGODENDROCYTE PRECURSORS

描述：本项目的目的是描述 GRO-α是一种α-趋化因子，最近被米勒博士的实验室证实， 调节少突胶质细胞反应的主要环境因素 PDGF的前体 在第一个具体目标中，趋化因子结构 增强PDGF增殖反应所需的基序 将定义少突胶质细胞前体。 在第二个具体目标中， 申请人打算建立最佳条件， GRO-α和PDGF诱导少突胶质细胞前体趋化性， GRO-α增强PDGF受体信号传导的能力。 第三 具体目的，GRO-α作用的分子机制将通过以下方式阐明： 评价配体结合特性，以及通过测定 钙和G蛋白参与信号传导级联反应。 在 第四个具体目标，生物有效的分布 将测定发育中的脊髓中的趋化因子。

DESCRIPTION: The purpose of this project is to characterize the role of GRO-a, an a-chemokine recently demonstrated by Dr. Miller's laboratory to be a major environmental factor that modulates the response of oligodendrocyte precursors to PDGF. In the first Specific Aim, the chemokine structural motifs required to enhance the PDGF proliferation response of oligodendrocyte precursors will be defined. In the second Specific Aim, the applicant intends to establish optimal conditions for interactions between GRO-alpha and PDGF to elicit oligodendrocyte precursor chemotaxis, and the capacity of GRO-alpha to enhance PDGF receptor signaling. In the third Specific Aim, molecular mechanisms of GRO-alpha action will be elucidated by evaluating ligand binding characteristics, as well as by determining the involvement of calcium and G proteins in signal transduction cascade. In the fourth Specific Aim, the distribution of biologically effective chemokines in the developing spinal cord will be determined.