OCULAR CONTROL OF MELATONIN REGULATION--ACTION SPECTRUM

为神经传递光刺激的光感受器系统- 光的内分泌、昼夜节律和治疗效果尚不清楚。这个 本研究的总体目标是确定 调节光的这些生物学和治疗效果。要实现 这个目标，一个行动谱(不同的相对效力 激发生物反应的波长)将被确定为 帮助识别光感受器系统对光的调节 人类体内的激素褪黑激素。然后，该动作谱可用于 一种研究动作光谱和相关光感受器的工具 参与昼夜节律和光疗的系统。 提议的研究的具体目的是确定一项行动 光诱导血浆褪黑素抑制谱的研究 单色波长(10毫米半峰值带宽)在400至760之间 NM。这些波长对急性抑制效应的影响 褪黑激素将在正常志愿者身上进行研究。通量-响应曲线 覆盖5个对数单位的光子密度，范围从10&lt；14；到10&lt；19&gt； 将为每个人建立角膜水平的光子/cm；2 波长。动作谱将通过绘制 半饱和刺激(sigma；-1)的倒数 测试了8个波长。这一动作谱将澄清范围和 将光刺激传输到的受体系统的峰值灵敏度 人类神经内分泌和昼夜节律系统。

The photoreceptor system that transduces light stimuli for neuro- endocrine, circadian, and therapeutic effects of light is unknown. The overall aim of this research is to determine the receptor mechanism that mediates these biological and therapeutic effects of light. To achieve this aim, an action spectrum (the relative effectiveness of different wave lengths for eliciting a biological response) will be established to help identify the photoreceptor system for light regulation of the hormone melatonin in humans. This action spectrum may then be used as a tool for investigating the action spectrum and related photoreceptor system involved in circadian regulation and light therapy. The specific aim of the proposed research is to determine an action spectrum for light-induced plasma melatonin suppression using 8 monochromatic wavelengths (10mm half-peak bandwidths) between 400 to 760 NM. The effects of these wavelengths on the acute suppression of melatonin will be studied in normal volunteers. fluence-response curves covering a 5 log unit photon density range from 10<14> to 10<19> photons/cm<2> at the level of the corneal will be established for each wavelength. an action spectrum will be constructed by plotting the reciprocal of the half-saturation stimulus (sigma<-1>) from each of the 8 wavelengths tested. This action spectrum will clarify the range and peak sensitivity of the receptor system that transmits photic stimuli to the human neuroendocrine and circadian system.