CORE--DNA SEQUENCING

DNA测序核心设施一直是 NEOMAMDC自1993年以来，大大加强了研究 中心的基础设施，并加快关节炎的进展 研究人员虽然分子生物学程序的范围， 与关节炎中心相关的实验室非常广泛， NEOMAMDC的大多数实验室都受益于特定的项目， 将继续受益于致力于DNA测序的核心设施 保持战略定力其基本原理是：1）DNA测序是 在任何以分子生物学为导向的实验中， 方法，以及2）每个实验室并不总是具有成本效益， 建立个人协议，购买设备，培训必要的 人员的3)DNA序列分析是劳动和时间密集型的，但 不需要对大型项目有具体的了解。4)酒店内的 测序设施提高了关节炎中心的整体生产力 通过更有效地执行这项任务， 5）比单独使用更高的精度 核心设施为调查人员腾出时间， 追求 核心的主要目标是协助那些调查人员已经 从事分子生物学研究为了实用， 不用于执行除DNA以外任何分子生物学技术 序列分析研究者负责准备 在提交之前进行测序的样本。样品方案 由测序核心提供制备，以确保 核心设施将得到有效利用，并确保成功。在 一般来说，研究人员负责将DNA片段连接到 通常使用的测序载体的多接头（克隆位点）。的 然后核心设施负责进行测序反应， 运行自动测序仪器并记录序列 一份可供调查人员进一步分析的文件。 核心的第二个目标是作为基因组DNA的储存库 来自患有OA的家庭成员，显示出家族遗传， 和来自OA疾病亚组的患者。这些人的DNA 在合作的基础上提供给其他调查人员。 此外，核心功能是筛选这个DNA库中的突变， II型胶原基因

The DNA sequencing core facility has been an important component of the NEOMAMDC since 1993, and has significantly enhanced the research infrastructure of the Center, and accelerated progress for Arthritis researchers. Although the scope of molecular biology procedures used by laboratories associated with the Arthritis Center is quite broad and are project-specific most laboratories of the NEOMAMDC have benefitted and will continue to benefit from a core facility dedicated to DNA sequence determination. The rationale for this is that 1) DNA sequencing is required at some point in any molecular biology oriented experimental approach, and 2) it is not always cost effective for each laboratory to set up individual protocols, purchase equipment an train the necessary personnel. 3) DNA sequence analysis is labor and time intensive, but does not require specific knowledge of the large project. 4) The on site sequencing facility enhances the overall productivity of Arthritis Center research laboratories by performing this task more efficiently and with greater precision than would be possible on an individual basis 5) The core facility frees time for investigators to engage in more creative pursuits. The primary objective of the core is to assist those investigators already engaged in molecular biology protocols. In order to be practical, it does not serve to perform any molecular biology technique other than DNA sequence analysis. Investigators are responsible for preparation of samples for sequencing in advance of submission. Protocols for samples preparation are provided by the sequencing core in order to assure that the core facility will be efficiently utilize and to assure success. In general, investigators are responsible for ligation of DNA fragments into the polylinker (cloning site) of sequencing vectors commonly in use. The core facility is then responsible for performing the sequencing reactions, running the automated sequencing instrument and recording the sequence as a file that can be used for further analysis by the investigator. The second objective of the core is to serve as repository for genomic DNA from members of families afflicted with OA showing familial inheritance, and from patients with OA disease subsets. DNA from these individuals is made available to other investigators on a collaborative basis. Additionally, the core functions to screen this DNA bank for mutations in the type II collagen gene.