CORE--DNA SEQUENCING

核心——DNA测序

• 批准号: 6324602
• 负责人: THOMAS Martin HERING
• 金额: $ 3.83万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6324602
• 关键词: DNA; biomedical facility; family genetics; human genetic material tag; human tissue; nucleic acid sequence; osteoarthritis; tissue resource /registry

The DNA sequencing core facility has been an important component of the NEOMAMDC since 1993, and has significantly enhanced the research infrastructure of the Center, and accelerated progress for Arthritis researchers. Although the scope of molecular biology procedures used by laboratories associated with the Arthritis Center is quite broad and are project-specific most laboratories of the NEOMAMDC have benefitted and will continue to benefit from a core facility dedicated to DNA sequence determination. The rationale for this is that 1) DNA sequencing is required at some point in any molecular biology oriented experimental approach, and 2) it is not always cost effective for each laboratory to set up individual protocols, purchase equipment an train the necessary personnel. 3) DNA sequence analysis is labor and time intensive, but does not require specific knowledge of the large project. 4) The on site sequencing facility enhances the overall productivity of Arthritis Center research laboratories by performing this task more efficiently and with greater precision than would be possible on an individual basis 5) The core facility frees time for investigators to engage in more creative pursuits. The primary objective of the core is to assist those investigators already engaged in molecular biology protocols. In order to be practical, it does not serve to perform any molecular biology technique other than DNA sequence analysis. Investigators are responsible for preparation of samples for sequencing in advance of submission. Protocols for samples preparation are provided by the sequencing core in order to assure that the core facility will be efficiently utilize and to assure success. In general, investigators are responsible for ligation of DNA fragments into the polylinker (cloning site) of sequencing vectors commonly in use. The core facility is then responsible for performing the sequencing reactions, running the automated sequencing instrument and recording the sequence as a file that can be used for further analysis by the investigator. The second objective of the core is to serve as repository for genomic DNA from members of families afflicted with OA showing familial inheritance, and from patients with OA disease subsets. DNA from these individuals is made available to other investigators on a collaborative basis. Additionally, the core functions to screen this DNA bank for mutations in the type II collagen gene.

DNA测序核心设施一直是 自1993年以来，NeOMAMDC已经大大加强了这项研究 该中心的基础设施，并加快了关节炎的进展 研究人员。尽管分子生物学程序使用的范围 与关节炎中心有关的实验室相当广泛，而且 具体项目NEOMAMDC的大多数实验室都受益于 将继续受益于致力于DNA序列的核心设施 决心。这样做的理由是：1)DNA测序 在任何以分子生物学为导向的实验中的某个点上都需要 方法，以及2)每个实验室并不总是具有成本效益 建立个人协议，购买必要的设备和培训 人事部。3)DNA序列分析是劳动和时间密集型的，但 不需要对大型项目有专门的了解。4)现场 测序设备提高了关节炎中心的整体生产率 通过更高效地执行这项任务并通过 比个人可能达到的更高精度5) 核心设施为调查人员腾出时间从事更具创造性的工作 追求。 该核心的主要目标是协助那些已经调查的人员 从事分子生物学方案的研究。为了切合实际，它确实是 不用于执行除DNA之外的任何分子生物学技术 序列分析。调查人员负责准备 样品在提交前进行排序。关于样品的协议 由测序核心提供准备，以确保 核心设施将得到有效利用，并确保成功。在……里面 将军，调查员负责将DNA片段连接到 常用测序载体的多联体(克隆位点)。这个 核心设备然后负责执行测序反应， 运行自动测序仪并将序列记录为 调查员可用于进一步分析的文件。 核心的第二个目标是作为基因组DNA的储存库 来自表现出家族遗传的患有骨性关节炎的家庭成员， 以及来自骨性关节炎疾病亚群的患者。这些人的DNA是 在合作的基础上提供给其他调查人员。 此外，核心功能是筛选这个DNA库中的突变 II型胶原基因。