SEARCH FOR NOVEL B CELL HYPERACTIVITY GENES

寻找新型 B 细胞过度活跃基因

• 批准号: 6100391
• 负责人: SYAMAL K DATTA
• 金额: $ 15.9万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6100391
• 关键词: B lymphocyte; CD5 molecule; animal genetic material tag; apoptosis; biological signal transduction; cell differentiation; complementary DNA; flow cytometry; gene expression; genetic strain; immunogenetics; laboratory mouse; leukocyte activation /transformation; messenger RNA; molecular cloning; northern blottings; nucleic acid sequence; polymerase chain reaction; receptor expression; systemic lupus erythematosus

Our long-term goal is to identify the genes and their products that determine hyperactivity of B cells, particularly B-1 (DC5-lineage) B cells. New Zealand Black (NZB) mice, that have a life-long hyperactivity ob B-1 cells, will be studied. NZB mice not only develop autoimmune disease but also a high incidence of malignant lymphomas of B-1 B cells that are very similar to human CLL and the B cell lymphomas developing the AIDS patients. In addition B-1 B cells are hyperactive in the inflamed joints of rheumatoid arthritis (RA) patients where they play a major role in antigen presentation and activation of inflammatory cells. We have preliminary evidence showing that the hyperactive B cells of NZB mice differentially express several genes that are not expressed in normal B cells. In this development and feasibility study, we would like to confirm the identity of these B cell hyperactivity genes. Eventually we aim to clone the full length cDNA for these differentially expressed genes, sequence them, study their genomic organization and define their function. This search for B cell hyperactivity genes may reveal novel genes or new function for known genes. Besides its relevance to RA and malignant B cell lymphomas as stated above, this project could enhance our understanding of the basic mechanisms of B cell differentiation, signal transduction, tolerance and apoptosis.

我们的长期目标是确定基因和它们的产物