PHYSIOLOGY OF THE DEVELOPING OLFACTORY EPITHELIUM

发育中的嗅觉上皮的生理学

• 批准号: 6174932
• 负责人: William Craig Michel
• 金额: $ 18.13万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-01-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6174932
• 关键词: action potentials; alternatives to animals in research; biological signal transduction; calcium channel; chemoreceptors; developmental neurobiology; electrical conductance; electrical potential; electrophysiology; membrane channels; membrane potentials; neural conduction; neural information processing; neural plasticity; neurons; odors; olfactions; olfactory stimulus; olfactory threshold; receptor sensitivity; second messengers; sensory mechanism; stimulus /response; voltage gated channel; zebrafish

DESCRIPTION: (Adapted From the Applicant's Abstract) Olfaction provides critical information to the very young. Olfactory cues guide neonates to feed and permit siblings to be recognized from other conspecifics. Imprinting on maternal or environmental cues occurs early in life when the olfactory system is rapidly developing. Numerous, anatomical, immunocytochemical and molecular genetic studies have advanced out understanding of the changes occurring during early development. Physiological studies, using activity dependent markers, have demonstrated the essential roles of the olfactory bulb and higher olfactory centers in preference learning and imprinting. There is, however, little information about the basic physiological development of the olfactory epithelium or the changes that might occur during preference learning or imprinting. We propose to functionally characterize the developing olfactory epithelium of the zebrafish using conventional electrophysiological and calcium imaging methods and a novel activity-dependent immunolabeling technique. This emerging developmental model was selected because it breeds year- round, fertilization and development occur externally and rapidly and large numbers of time-staged embryos can be maintained. Our first aim is to determine when after fertilization the voltage, calcium and second messenger-gated currents required to transduce an odorant-binding event into an action potential develop. Our second and third aims characterize the temporal development of odor sensitivity and the spatial distribution of odor-stimulated olfactory receptor neurons (ORNs). Odor-stimulated ORNs will be labeled with an ion channel permeant probe, 1-amino-4- guanidobutane (AGB), and anti-AGB IgG antibodies. Complementary electrophysiological and calcium imaging studied will characterize the response properties of developing ORNs in situ and individually. The final aim is to determine the role odor environment play in the functional development of the olfactory epithelium. The studies proposed will help identify the critical periods in the functional development of the olfactory epithelium and may provide insight into the relative roles of environment versus programmed genetic events in normal developmental processes.

描述：（根据申请人的摘要改编）嗅觉提供 非常年轻的关键信息。嗅觉提示引导新生儿 饲料和允许兄弟姐妹可以从其他同种特定中识别出来。 当孕产妇或环境提示上的印记在生命的早期就发生 嗅觉系统正在迅速发展。许多，解剖学， 免疫细胞化学和分子遗传学研究已促进 了解早期发展过程中发生的变化。 使用依赖性标记的生理研究已证明 嗅球和较高嗅觉中心的重要作用 偏好学习和印迹。但是，很少有信息 关于嗅觉上皮的基本生理发展或 偏好学习或印迹期间可能发生的变化。我们 建议在功能上表征发展中的嗅觉上皮 使用常规电生理学和钙的斑马鱼 成像方法和一种新型活性依赖性免疫标记技术。 之所以选择这种新兴发展模型，是因为它繁殖了一年 圆形，受精和发育在外部和快速发生 可以维持大量时间阶段的胚胎。我们的第一个目标是 确定施肥后何时钙，钙和第二 传递气味结合事件所需的使者门控电流 发展到动作潜力。我们的第二和第三目标是 气味灵敏度和空间分布的时间发展 气味刺激的嗅觉受体神经元（ORNS）。气味刺激的ORN 将用离子通道渗透探针标记1-氨基-4-- 鸟甲酰丁烷（AGB）和抗AGB IgG抗体。补充 所研究的电生理和钙成像将表征 开发ORN的原位和单独的响应属性。决赛 目的是确定气味环境在功能中的作用 嗅觉上皮的发展。提出的研究将有助于 确定功能发展的关键时期 嗅觉上皮，并可以深入了解 正常发育中的环境与编程的遗传事件 过程。