COCHLEAR HAIR CELL TRANSDUCTION

设计了多种实验来考察两者的运行情况 哺乳动物听觉器官感觉感受器细胞的类型：内毛 细胞和外毛细胞。 现在我们知道内毛细胞 将听觉信息传递给大脑，而外毛细胞 改变耳蜗中的机械环境， 放大 在这项工作中， 细胞内从毛细胞和细胞外为他们的直接 麻醉豚鼠Corti器官内的环境。 一 拟议工作的很大一部分是为了调查 单个外毛细胞受体电位（它们的 电反应的声音）和总细胞外电 应答 建议后者可能会影响放大 以高频率进行处理。 此外，当代的描述是 寻求耳蜗电解剖学（其电阻抗模式）， 特别是在高频率下，以及Corti器官本身。 最后，我们的目标是继续研究旨在评估耳蜗 非线性过程（双音抑制，低频偏置， 结合音调和谐波产生）， 以及受沿着耳蜗的纵向位置的影响 螺旋形。 除了描述耳蜗的内在兴趣之外， 非线性处理，这项工作的大部分也用于诊断， 评估内毛细胞与外毛细胞的功能， 细胞外反应 这些研究的一个子集旨在最终 解决了细胞内生产长期存在的差异， 紧张性感受器电位，由高水平的外毛细胞产生， 与耳蜗的低频区域相比。

A variety of experiments are designed to examine the operation of the two types of sensory receptor cells of the mammalian hearing organ: inner hair cells and outer hair cells. It is now known that inner hair cells communicate auditory information to the brain, whereas outer hair cells modify the mechanical environment in the cochlea thereby producing amplification. In this work electrical responses to sound are recorded intracellularly from hair cells and extracellularly for their immediate environment in the organ of Corti of anesthetized guinea pigs. A significant portion of the proposed work is aimed at an investigation of the connections between single outer hair cell receptor potentials (their electrical response to sound) and gross extracellular electrical responses. It is proposed that the latter may influence the amplification process at high frequencies. Further, contemporary descriptions are sought of cochlear electroanatomy (its electrical impedance pattern), particularly at high frequencies, and of the organ of Corti proper. Finally, we aim to continue studies designed to evaluate cochlear nonlinear processes (two-tone suppression, low-frequency biasing, combination tone and harmonic production) as reflected in receptor potentials and as influenced by longitudinal location along the cochlear spiral. Aside from its intrinsic interest in describing cochlear nonlinear processing, much of this work is also used diagnostically to evaluate inner versus outer hair cell function and the sources of extracellular responses. A subset of these studies is aimed at finally resolving the long-standing discrepancy in the production of intracellular tonic receptor potentials, generated by outer hair cells in the high versus low frequency regions of the cochlea.