COCHLEAR HAIR CELL TRANSDUCTION

耳蜗毛细胞传导

• 批准号: 6174991
• 负责人: PETER J DALLOS
• 金额: $ 32.12万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6174991
• 关键词: biological signal transduction; cochlear microphonic potentials; ear hair cell; electrophysiology; guinea pigs; membrane potentials; sound frequency

A variety of experiments are designed to examine the operation of the two types of sensory receptor cells of the mammalian hearing organ: inner hair cells and outer hair cells. It is now known that inner hair cells communicate auditory information to the brain, whereas outer hair cells modify the mechanical environment in the cochlea thereby producing amplification. In this work electrical responses to sound are recorded intracellularly from hair cells and extracellularly for their immediate environment in the organ of Corti of anesthetized guinea pigs. A significant portion of the proposed work is aimed at an investigation of the connections between single outer hair cell receptor potentials (their electrical response to sound) and gross extracellular electrical responses. It is proposed that the latter may influence the amplification process at high frequencies. Further, contemporary descriptions are sought of cochlear electroanatomy (its electrical impedance pattern), particularly at high frequencies, and of the organ of Corti proper. Finally, we aim to continue studies designed to evaluate cochlear nonlinear processes (two-tone suppression, low-frequency biasing, combination tone and harmonic production) as reflected in receptor potentials and as influenced by longitudinal location along the cochlear spiral. Aside from its intrinsic interest in describing cochlear nonlinear processing, much of this work is also used diagnostically to evaluate inner versus outer hair cell function and the sources of extracellular responses. A subset of these studies is aimed at finally resolving the long-standing discrepancy in the production of intracellular tonic receptor potentials, generated by outer hair cells in the high versus low frequency regions of the cochlea.

设计了各种实验来检验这两种方法的运行情况 哺乳动物听力器官的感觉感受器细胞类型：内毛 细胞和外毛细胞。现已知，内毛细胞 将听觉信息传递给大脑，而外毛细胞 改变耳蜗内的机械环境，从而产生 放大。在这项工作中，记录了对声音的电响应 细胞内的毛细胞和细胞外的毛细胞 麻醉豚鼠Corti器官的环境。一个 拟议工作的很大一部分旨在调查 单个外毛细胞受体电位之间的联系 对声音的电反应)和总的细胞外电 回应。有人认为后者可能会影响扩增。 以高频进行处理。此外，当代的描述是 寻求耳蜗电解剖(其阻抗模式)， 尤其是在高频下，以及科尔蒂本身的器官。 最后，我们的目标是继续进行旨在评估耳蜗的研究 非线性过程(双音抑制、低频偏置、 合音和和声的产生)反映在感受器中 电位和AS受耳蜗轴向位置的影响 螺旋形。除了它对描述耳蜗的内在兴趣之外 非线性处理，这项工作的大部分也被用于诊断 评估内毛细胞和外毛细胞的功能和来源 细胞外反应。这些研究的一个子集旨在最终 解决细胞内生产中长期存在的差异 紧张性感受器电位，由外毛细胞在高 与耳蜗低频区的对比。