CORE--ANALYTICAL REAGENTS FACILITY

核心——分析试剂设施

• 批准号: 6099471
• 负责人: FRANK W. FITCH
• 金额: $ 14.48万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6099471
• 关键词: biochemistry; biomedical facility; enzyme linked immunosorbent assay; flow cytometry; polymerase chain reaction; reagent /indicator

This core will provide the various projects with quality controlled analytical reagents for cytokine ELISA's, Flow Cytometry and Biochemistry (for Signal Transduction) and Molecular Biology (for Quantitative-PCR of cytokines). The task of this core is to provide in a very cost effective way analytical reagents common for the various project. This will be achieved by performing many labor intensive routine quality control assays otherwise done in the individual laboratories of the program project. Among the assays are titrations and quality controls of common commercial reagents which in the past have shown many batch to batch variables, like antibodies preparations for ELISA, FACS and Western Blot.

该核心将为各种项目提供质量控制 用于细胞因子 ELISA、流式细胞术和生物化学的分析试剂 （用于信号转导）和分子生物学（用于定量​​ PCR 细胞因子）。该核心的任务是提供一种非常具有成本效益的 方式分析试剂通用，适用于各个项目。这将是 通过执行许多劳动密集型常规质量控制测定来实现 否则在该计划项目的各个实验室进行。 检测方法包括常见商业产品的滴定和质量控制 过去显示出许多批次间变量的试剂，例如 用于 ELISA、FACS 和 Western Blot 的抗体制剂。