CORE--ANALYTICAL REAGENTS FACILITY

核心——分析试剂设施

• 批准号: 6234971
• 负责人: FRANK W. FITCH
• 金额: $ 10.03万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6234971
• 关键词: biochemistry; biomedical facility; enzyme linked immunosorbent assay; flow cytometry; polymerase chain reaction; reagent /indicator

This core will provide the various projects with quality controlled analytical reagents for cytokine ELISA's, Flow Cytometry and Biochemistry (for Signal Transduction) and Molecular Biology (for Quantitative-PCR of cytokines). The task of this core is to provide in a very cost effective way analytical reagents common for the various project. This will be achieved by performing many labor intensive routine quality control assays otherwise done in the individual laboratories of the program project. Among the assays are titrations and quality controls of common commercial reagents which in the past have shown many batch to batch variables, like antibodies preparations for ELISA, FACS and Western Blot.

这个核心将提供各种项目的质量控制 细胞因子ELISA、流式细胞术和生化分析试剂 (for信号转导）和分子生物学（用于定量PCR） 细胞因子）。这个核心的任务是提供一个非常具有成本效益的 方法分析试剂通用于各种项目。这将是 通过执行许多劳动密集型常规质量控制测定来实现 否则在项目的各个实验室进行。 在这些测定中，有常见的商业化的滴定和质量控制。 试剂在过去显示出许多批次间变量，如 用于ELISA、FACS和Western印迹的抗体制备。