CORE--TRANSGENIC MOUSE AND FACS FACILITY

这个应用程序的核心由两个主要组件组成。在 第一个构成部分，请求为继续运作提供支助 转基因小鼠设施，已成为该计划的组成部分 从一开始就是这样。该设施提供胚胎显微注射。 以及产生转基因小鼠所需的手术； 产生假孕受体小鼠用于注射小鼠的植入 胚胎；并在注射怀孕的方案中提供帮助 马血清和绒毛膜促性腺激素用于超数排卵。在.期间 在过去的五年里，这个设施得到了广泛的使用。 麦克德维特。戴维斯、魏斯曼和帕恩斯，未来五年将 由这些调查人员以及琼斯博士和古德诺博士在 核心建议中描述的一系列转基因小鼠品系。 核心设备的第二个组件是被激活的荧光 细胞分类器，它为所有实验室提供支持 这个项目中的调查人员。这是最先进的设施，由 赫森伯格博士和帕克斯。它包括两个多参数FACS分类器。 四参数FACSSCAN流式细胞仪及其扩展计算 分析来自分析运行的数据所需的设施 在分拣机上。这是一种独特的资源，它提供了极好的 向该计划中的所有实验室以及 斯坦福医学院的其他一些实验室。这个设施 在这笔赠款上，调查人员使用了大量的资金 的拟议项目需要持续的高使用率的实验 描述。

The Core of this application consists of two major components. In the first component, support is requested for continued operation of the transgenic mouse facility which has been an integral part of this program since its inception. This facility provides for embryo microinjections and the surgery required for generating transgenic mice;, breeds and produces pseudopregnant recipient mice for implantation of injected murine embryos; and provides help in the protocols for injections of pregnant mare's serum and chorionic gonadotrophin for superovulation. During the past five years, this facility, has been extensively used by Drs. McDevitt. Davis, Weissman and Parnes and during the coming five years will be used by these investigators as well as by Drs. Jones and Goodnow in a series of transgenic mouse lines described in the Core proposal. The second component of the Core facility is the fluorescence activated cell sorter, which provides support to the laboratories of all the investigators in this program. This is a state of the art facility run by Drs. Herzenberg and Parks. It includes two multiparameter FACS sorters. a four parameter FACSscan flow cytometer and extensive computing facilities required for the analysis of data coming from analytical runs on the sorter. This is a unique resource which has provided excellent technical support to all of the laboratories in the program as well as to a number of other laboratories at Stanford Medical School. This facility has used been used very heavily by the investigators on this grant and all of the proposed projects require continued high usage for the experiments described.