CORE--CHLAMYDIA

沙眼衣原体实验室核心将：1)提供纯化的 微生物，进行微生物和DNA扩增检测 方法，并测定项目1的血清和局部抗体水平 (首席研究员多萝西·巴顿)；2)进行体外MIC和MBC 用开发的或新型的抗菌化合物进行抗菌检测。 项目2沙眼衣原体(Charles lsaacs，P.I.)；3)感染器官型 用沙眼衣原体进行组织培养并测定其MIC水平 项目3中抗衣原体化合物的“筏”培养(詹姆斯) McDougall，P.I.)；以及4)评估新型抗肿瘤药物的作用方式 在这些研究中确定的衣原体化合物。沙眼衣原体 实验室核心将执行高度专业化的微生物学 衣原体检测方法和血清学分析技术 这是常规微生物学实验室所不具备的。自.以来 实验室核心一直在完整地参与开发和 沙眼衣原体DNA扩增方法的应用 实验室已经通过这些非常新的技术获得了相当多的专业知识 技术。实验室的核心还进行了MIC和MBC 针对衣原体的各种抗生素的测定，也正在建立 在这个非常专业的领域有很多专业知识。所有这些技术都是 对于此应用程序中的几个项目及其 核心实验室的规定将确保这些规定将得到实施 由技术经验丰富的人员以经济的方式进行。

The Chlamydia trachomatis laboratory core will: 1) provide purified organisms, perform microbiological and DNA amplification detection methods, and determine serum and local antibody levels for Project 1 (Dorothy Patton, Principal Investigator); 2) perform in vitro MIC and MBC assays with developed or novel antimicrobial compounds against C. trachomatis for Project 2 (Charles lsaacs, P.I.); 3) infect organotypic tissue cultures with C. trachomatis and determine MIC levels in these "raft" cultures for anti-chlamydial compounds for Project 3 (James McDougall, P. I.); and 4) assess the mode of action of novel anti- chlamydial compounds identified in these studies. The C. trachomatis laboratory core will perform the highly specialized microbiological detection methods and serological analysis for chlamydia, techniques which are not available in routine microbiology laboratories. Since the laboratory core has been integrally involved with the development and application of DNA amplification methods for C. trachomatis, this laboratory has gained considerable expertise with these very new technologies. The laboratory core has also carried out MIC and MBC determinations for various antibiotics against chlamydia, also building much expertise in this very specialized area. All of these techniques are essential for several of the projects in this application and their provision in a core laboratory will ensure that they will be carried out by technically experienced personnel in an economical manner.