CORE--CHLAMYDIA

沙眼衣原体实验室核心将：1）提供纯化的 微生物，进行微生物和DNA扩增检测 方法，并确定项目1的血清和局部抗体水平 （Dorothy Patton，主要研究者）; 2）进行体外MIC和MBC 用开发的或新的抗微生物化合物对C. 项目2的沙眼（Charles lsaacs，P.I.）; 3)感染器官型 组织培养，C.沙眼衣原体，并确定MIC水平在这些 用于项目3的抗衣原体化合物的“筏”培养物（James McDougall，P. I.）;和4）评估新型抗- 这些研究中鉴定出的衣原体化合物。梭沙眼衣原体 实验室核心将执行高度专业化的微生物 衣原体的检测方法和血清学分析，技术 这是常规微生物实验室所不能提供的。以来 实验室核心一直参与开发和 DNA扩增技术在C.沙眼，这 实验室已经获得了相当多的专业知识，这些非常新的 技术.实验室核心还进行了MIC和MBC 对衣原体的各种抗生素的测定，也建立 在这个非常专业的领域有很多专业知识。所有这些技术都 对于本申请中的几个项目及其 在核心实验室提供将确保他们将进行 由技术经验丰富的人员以经济的方式。

The Chlamydia trachomatis laboratory core will: 1) provide purified organisms, perform microbiological and DNA amplification detection methods, and determine serum and local antibody levels for Project 1 (Dorothy Patton, Principal Investigator); 2) perform in vitro MIC and MBC assays with developed or novel antimicrobial compounds against C. trachomatis for Project 2 (Charles lsaacs, P.I.); 3) infect organotypic tissue cultures with C. trachomatis and determine MIC levels in these "raft" cultures for anti-chlamydial compounds for Project 3 (James McDougall, P. I.); and 4) assess the mode of action of novel anti- chlamydial compounds identified in these studies. The C. trachomatis laboratory core will perform the highly specialized microbiological detection methods and serological analysis for chlamydia, techniques which are not available in routine microbiology laboratories. Since the laboratory core has been integrally involved with the development and application of DNA amplification methods for C. trachomatis, this laboratory has gained considerable expertise with these very new technologies. The laboratory core has also carried out MIC and MBC determinations for various antibiotics against chlamydia, also building much expertise in this very specialized area. All of these techniques are essential for several of the projects in this application and their provision in a core laboratory will ensure that they will be carried out by technically experienced personnel in an economical manner.