CORE--CHLAMYDIA

核心--衣原体

• 批准号: 6352620
• 负责人: MARY F LAMPE
• 金额: $ 5.83万
• 依托单位: MAGEE-WOMEN'S HOSPITAL OF UPMC
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-15 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6352620
• 关键词: Chlamydiaceae; antibiotics; biomedical facility; drug screening /evaluation; microorganism culture; microorganism resource /registry

Chlamydia trachomatis is the most common sexually-transmitted bacterial pathogen worldwide, causing millions of new infections each year. These infections all too frequently result in serious sequelae in women, including pelvic inflammatory disease, infertility, ectopic pregnancy, and chronic pelvic pain. Although screening programs can reduce the prevalence of chlamydial infections and their complications, primary prevention programs are needed. The use of topical microbicides has been recognized as a productive approach for the prevention of chlamydial infections and STD's in general. Chlamydiae are obligate intracellular bacteria which can only grow within eukaryotic cells. Infectious elementary bodies are found in sexual secretions and are adapted for survival in this environment. The organisms are only extracellular for a brief period of their life cycle, but this extra cellular phase is essential for their transmission from person to person. It is during this brief time that Chlamydia are amenable to killing by topical microbicides. The overall goal of this proposal is to evaluate various topical microbicides for their activity against C. trachomatis. This includes: 1. Providing purified C. trachomatis organisms and performing microbiological and DNA amplification methods of chlamydial detection 2. Performing in vitro minimum cidal concentration (MCC) assays with developed or novel antimicrobial compounds and with excipients to determine their activity against C. trachomatis, and 3. assessing the mode of action of previously identified active compounds on C. trachomatis. The Chlamydia Core will provide purified organisms and highly specialized microbiological detection methods for Chlamydia, materials and techniques available in only a few laboratories in the United States. In addition, the Chlamydia Core will carry out MCC assays with various microbicides against C. trachomatis, utilizing our unique expertise with this highly specialized test. Finally, we will examine the mode of action of several microbicides on this organism, providing the first information about how these microbicides exert their anti-chlamydial effects.

沙眼衣原体是全世界最常见的性传播细菌病原体，每年造成数百万新感染。这些感染经常导致妇女严重的后遗症，包括盆腔炎、不孕症、异位妊娠和慢性盆腔疼痛。虽然筛查计划可以减少衣原体感染及其并发症的患病率，但需要一级预防计划。使用局部杀微生物剂被认为是预防衣原体感染和一般性性病的有效方法。衣原体是专性细胞内细菌，只能在真核细胞内生长。 在性分泌物中发现了感染性原体，它们适应于在这种环境中生存。这些生物只是在其生命周期的一个短暂时期内存在于细胞外，但这一细胞外阶段对于它们在人与人之间的传播至关重要。正是在这短暂的时间内，衣原体易于被局部杀微生物剂杀死。本提案的总体目标是评价各种局部杀微生物剂对C.沙眼这包括：1.得到纯化的C.沙眼病原体及衣原体的微生物和DNA扩增检测方法2.使用已开发或新型抗菌化合物和辅料进行体外最低杀菌浓度（MCC）测定，以确定其对念珠菌的活性。沙眼，和3.评估先前鉴定的活性化合物对C.沙眼衣原体核心将提供纯化的微生物和高度专业化的衣原体微生物检测方法，材料和技术在美国只有少数实验室可用。 此外，衣原体核心将进行MCC检测与各种杀微生物剂对C。沙眼，利用我们独特的专业知识与这种高度专业化的测试。最后，我们将研究几种杀微生物剂对这种微生物的作用方式，提供有关这些杀微生物剂如何发挥其抗衣原体作用的第一个信息。