CELLULAR AND MOLECULAR BIOLOGY OF THE HEPATIC STEM CELL COMPARTMENT

这项研究的重点是定义细胞和分子生物学 肝和胰腺干细胞分室的正常和 肿瘤器官，特别强调生长的作用 因子和细胞因子。进行本研究是为了确定 未成熟的胆汁上皮对生长的反应是否不同 胆汁淤积对成年动物的刺激作用。在……里面 此外，生长因子/受体系统的可能参与 与胆管内肝干细胞早期激活有关 检测细胞增殖情况。甲胎蛋白是高度选择性的 青年大鼠胆管结扎后7天小胆管中的表达 5周龄以下的大鼠。虽然没有显著增加，但 干细胞因子、c-kit、肝细胞生长因子和 在成年大鼠中观察到转化生长因子-α， 所有这些生长因子在胆管中的表达均增加。 结扎大鼠至5周龄。这些结果表明胆汁 幼年大鼠的上皮对胆汁淤积的反应方式是 表型与肝脏早期激活时所见相似 成人肝脏中的干细胞。

The research is focused on defining the cellular and molecular biology of the hepatic and pancreatic stem cell compartments in normal and neoplastic organs with a particular emphasis on the role of growth factors and cytokines. The present study was undertaken to determine whether the immature bile epithelium responds differently to growth stimulus induced by bile stasis to that seen in the adult animal. In addition, the possible involvement of the growth factor/receptor systems associated with early activation of hepatic stem cells in bile duct proliferation was examined. Alpha-fetoprotein was highly and selectively expressed in small bile ducts 7 days after bile duct ligation in young rats up to 5 weeks of age. Although no significant increase in the expression of stem cell factor, c-kit, hepatocyte growth factor and transforming growth factor-alpha is observed in adults rats, the expression of all these growth factors was increased in the bile duct ligated rat up to 5 weeks of age. These results indicate that the bile epithelium in young rats responds to bile stasis in a fashion that is phenotypically similar to that seen during early activation of hepatic stem cells in adult liver.