CORE--MOLECULAR BIOLOGY

核心--分子生物学

• 批准号: 6235767
• 负责人: POJEN P CHEN
• 金额: $ 11.54万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-03-07 至 1998-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6235767
• 关键词: arthritis; biomedical equipment resource; biomedical facility; biomedical resource; data collection; molecular biology; molecular cloning; oligonucleotides; peptide chemical synthesis; technology /technique; transfection

The objective of the Molecular Biology Core unit will be to facilitate the timely utilization of molecular biology techniques by physicians and scientists in the Center. During the last four years, the core has evolved from three originally approved functions to the current six functions. The effort and performances of the core have won the praise of outside reviewers of this Center. Accordingly, we propose to continue all six services, each will be directed by an experienced investigator with specialized professional skill. Core functions will include: 1) To maintain and operative a share instrument facility, including an electroporator set, a Batch/Continuous Fermentator, an UVP gel documentation system, PCR machines, ultracentrifuges, and GCG Sequence Analysis Software Package. 2) To provide affordable and high quality sequencing service. Dr. Chan will provide this service with a semi-automated system (Applied Biosystem Inc. Model 373) at $10 per sequencing, which generally yields an average of 300-400 bp. 3) To provide assistance in applying the combinatorial library technique. The core will provide established libraries, assist new library construction, and provide hands-on technical assistance. 4) To provide assistance in applying transgenic and knock out methodologies. Dr. Tighe will assist investigators to initiate and perform transgenic mouse studies, and freeze and maintain frozen embryos of selected transgenic animals. 5) to provide assistance in conducting genetic studies of human autoimmune diseases. Dr. Nobori will assist investigators in performing linkage analyses and population genetics, positional cloning (i.e., reverse genetics), yeast artificial chromosome library screening and long-range mapping, and genome wide search for genetic factors associated with autoimmune diseases. 6) To provide technical assistance in basic molecular biology techniques and to organize the methodology seminars and user group meetings. Dr. Olee will teach basic techniques, and organize seminars and user group discussions on subjects of interest to investigators.

分子生物学核心单元的目标是促进 医生及时利用分子生物学技术 科学家在中心。 在过去的四年里，核心 从最初核准的三个职能发展到目前的六个职能 功能协调发展的核心的努力和表现赢得了 对本中心外部评审员的赞扬。 因此，我们建议 为了继续所有六项服务，每项服务都将由一位经验丰富的 具有专门专业技能的调查员。 核心职能将 包括：（1）维持及运作共用票据设施， 包括电穿孔器组、分批/连续发酵器、 UVP凝胶记录系统、PCR仪、超浓缩仪和 GCG序列分析软件包 2)提供 价格合理的高质量测序服务。 陈医生会 用半自动化系统提供这项服务（应用 生物系统公司型号373），每次测序10美元，通常 产生平均300-400 bp。 3)提供援助 应用组合文库技术。 核心将提供 建立图书馆，协助新图书馆建设，并提供 实际操作技术援助。 4)协助申请 转基因和敲除方法。 泰伊医生会协助 研究人员启动并进行转基因小鼠研究，以及 冷冻和保存选定的转基因动物的冷冻胚胎。 5)协助进行人类基因研究， 自身免疫性疾病 Nobori博士将协助调查人员 进行连锁分析和群体遗传学， 克隆（即，反向遗传学），酵母人工染色体文库 筛选和远程定位，以及全基因组搜索， 与自身免疫性疾病相关的遗传因素。 6)提供 提供基本分子生物学技术方面的技术援助， 组织方法研讨会和用户小组会议。 博士 Olee将教授基本技术，并组织研讨会和用户 就调查人员感兴趣的问题进行小组讨论。