CELL ISOLATION AND UPKEEP

神经干细胞和神经上皮肿瘤的分离和维持核心 将协调维持神经干细胞和肿瘤细胞 线 它还将协调这些细胞分布到各种 个别项目。 这个核心将维持一个冷冻库， 用于各种项目的神经外科标本。 胎儿，术后- 将在核心中收获纳塔尔和成体干细胞。 核心 将使用细胞培养技术来提供这些服务。 因为 每个项目都需要使用这些细胞，核心将 确保项目主要研究基质的质量控制 项目 通过1）标准细胞培养确保质量控制 维持低传代次数的技术，2）免疫组化 染色，3）RT-PCR带型，和4）定时怀孕的 从其收获胎儿细胞的动物。

The Neural Stem Cell and Neuroepithelial Tumor Isolation and Upkeep Core will coordinate maintenance of neural stem cells and neoplastic cell lines. It will also coordinate distribution of these cells to various individual projects. This core will maintain a library of frozen neurosurgical specimens for use in the various projects. Fetal, post- natal, and adult stem cells will be harvested in the core. The core will use cell culture techniques to provide these services. Because each of the projects requires the use of these cells, the core will ensure quality control of the primary research substrates of the program project. Quality control will be ensured by 1) standard cell culture techniques maintaining low passage number, 2) immunohistochemical staining, 3) RT-PCR banding patterns, and 4) timed pregnancies of the animals from which the fetal cells are harvested.