CELL ISOLATION AND UPKEEP

细胞隔离和维护

• 批准号: 6103127
• 负责人: N S LITOFSKY
• 金额: $ 17.2万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6103127
• 关键词: biomedical facility; cell line; embryo /fetus tissue /cell culture; embryonic stem cell; immunocytochemistry; laboratory mouse; neoplastic cell; neurons; polymerase chain reaction; tissue /cell culture

The Neural Stem Cell and Neuroepithelial Tumor Isolation and Upkeep Core will coordinate maintenance of neural stem cells and neoplastic cell lines. It will also coordinate distribution of these cells to various individual projects. This core will maintain a library of frozen neurosurgical specimens for use in the various projects. Fetal, post- natal, and adult stem cells will be harvested in the core. The core will use cell culture techniques to provide these services. Because each of the projects requires the use of these cells, the core will ensure quality control of the primary research substrates of the program project. Quality control will be ensured by 1) standard cell culture techniques maintaining low passage number, 2) immunohistochemical staining, 3) RT-PCR banding patterns, and 4) timed pregnancies of the animals from which the fetal cells are harvested.

神经干细胞和神经上皮肿瘤分离和维护核心 将协调神经干细胞和肿瘤细胞的维护 线。 它还将协调这些单元格的分布到各个 个人项目。 该核心将维护一个冻结的库 用于各种项目的神经外科标本。 胎儿、产后 出生和成体干细胞将在核心中收获。 核心 将使用细胞培养技术来提供这些服务。 因为 每个项目都需要使用这些单元，核心将 确保项目主要研究基质的质量控制 项目。 质量控制将通过 1) 标准细胞培养来确保 维持低传代数的技术，2) 免疫组织化学 染色，3) RT-PCR 条带模式，以及 4) 定时妊娠 从中收获胎儿细胞的动物。