CELL ISOLATION AND UPKEEP

神经干细胞与神经上皮性肿瘤的分离与维持核心 将协调神经干细胞和肿瘤细胞的维护 台词。它还将协调这些细胞分布到不同的 个别项目。这个核心将维护一个冻结的库 用于各种项目的神经外科标本。胎儿，后- 出生的和成体的干细胞将被收获在核心。其核心是 将使用细胞培养技术来提供这些服务。因为 每个项目都需要使用这些单元，核心将 确保项目主要研究底物的质量控制 项目。质量控制将由1)标准细胞培养来保证 保持低传代数的技术，2)免疫组织化学 染色，3)RT-PCR带型，以及4)定时怀孕 获取胎儿细胞的动物。

The Neural Stem Cell and Neuroepithelial Tumor Isolation and Upkeep Core will coordinate maintenance of neural stem cells and neoplastic cell lines. It will also coordinate distribution of these cells to various individual projects. This core will maintain a library of frozen neurosurgical specimens for use in the various projects. Fetal, post- natal, and adult stem cells will be harvested in the core. The core will use cell culture techniques to provide these services. Because each of the projects requires the use of these cells, the core will ensure quality control of the primary research substrates of the program project. Quality control will be ensured by 1) standard cell culture techniques maintaining low passage number, 2) immunohistochemical staining, 3) RT-PCR banding patterns, and 4) timed pregnancies of the animals from which the fetal cells are harvested.