MICROPLATE SCINTILLATION/LUMINESCENCE/FLOUR COUNTER

微孔板闪烁/发光/面粉计数器

• 批准号: 2503802
• 负责人: THOMAS S. KUPPER
• 金额: $ 13.36万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2503802
• 关键词: biomedical equipment; biomedical equipment purchase; luminescence; scintillation counter

We propose to establish a core facility at Brigham and Women's Hospital that would provide the laboratories of members of the user group with the capability of rapidly quantitating radioisotopic emissions, luminescence, absorption, and fluorescence from samples prepared in standard format 96 well microtiter plates. All of these capabilities would be provided by an integrated system combining a MicroBeta Trilux liquid scintillation and luminescence counter, a Tomtec 96-well plate harvester, and a Victor multilabel counter for fluorescence and photometric measurements (all components to be purchased from Wallac, Inc.). The core facility will be based in the Harvard Skin Disease Research Core Center (HSDRC) currently funded by NIAMS. The major users are primarily drawn from investigators that are currently affiliated with the HSDRC with laboratory space in the same building (Thom Research Building) in which the HSDRC laboratories are located. The major users come from the Departments of Medicine (Dermatology and Gastroenterology Divisions), and Surgery. The proposed system will incorporate features (such as multiple detectors within the counter and plate stackers) to provide rapid answers following unattended counting of microtiter plate- based assays. Interfacing of the proposed system to an existing ethernet network will allow the participating laboratories rapid and convenient access to their results. The major types of assays to be performed by members of the user group using the proposed core facility will be: (a) cell proliferation assays based on 3H-thymidine incorporation; (b) luminescent detection of luciferase as a reporter gene in transfection and transgenic mouse experiments; (c) 51Cr release assays for quantitation of cytotoxicity; (d) static adhesion assays using fluorescently labeled cells; (e) DNA quantitation by fluorometry; and (f) absorption measurements on enzyme immunoassays. Rapid and efficient counting of each of these assay types will enable users of the core facility to achieve a substantial boost in productivity and allow for timely completion of experiments. We also anticipate that the shared use of this instrumentation will provide new opportunities for interactions between investigators from the laboratories of the major users, which should in time lead to initiation of new interdisciplinary research projects.

我们建议在布里格姆妇女医院建立一个核心设施 这将为用户组成员的实验室提供 快速量化放射性同位素排放的能力， 样品的发光、吸收和荧光 标准格式96孔微滴定板。所有这些功能 将由结合MicroBeta Trilux的集成系统提供 液体闪烁和发光计数器，Tomtec 96孔板 收割机和维克托多标记荧光计数器 光度测量(所有部件均从Wallac购买， Inc.)。核心设施将设在哈佛皮肤病中心 目前由NIAMS资助的研究核心中心(HSDRC)。主要用户 主要来自调查人员，他们目前 与HSDRC的实验室空间在同一大楼内(Thom Research HSDRC实验室所在的建筑物)。主要用户 来自内科(皮肤科和胃肠科) 部门)和外科。拟议的系统将包含一些功能 (例如计数器和板式堆叠机内的多个探测器) 在无人值守的微量滴度计数后提供快速答案- 基础化验。建议的系统与现有系统的接口 以太网将使参与的实验室能够快速和 方便地访问他们的结果。主要的化验类型有 由用户组成员使用拟议的核心设施执行 将是：(A)基于~3H-胸腺嘧啶核苷的细胞增殖分析 (B)荧光素酶作为报告分子的发光检测 转基因和转基因小鼠实验中的基因；(C)51Cr的释放 细胞毒性的定量测定；(D)静态粘附法 使用荧光标记的细胞；(E)用荧光法进行DNA定量； 和(F)酶免疫测定法上的吸收测量。快速且 这些化验类型中的每一种的有效计数将使用户能够 核心设施可大幅提高生产效率，并允许 为了及时完成实验。我们还预计， 共享使用此工具将为以下方面提供新的机会 来自该专业实验室的研究人员之间的互动 用户，这应该及时导致新的跨学科的启动 研究项目。